Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 651425..652152 Replicon chromosome
Accession NZ_CP110814
Organism Escherichia coli strain THB42-F4

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag N0552_RS03185 Protein ID WP_000550189.1
Coordinates 651425..651739 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag N0552_RS03190 Protein ID WP_000560266.1
Coordinates 651736..652152 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N0552_RS03165 (647583) 647583..648569 - 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -
N0552_RS03170 (648648) 648648..649340 - 693 WP_000942548.1 vancomycin high temperature exclusion protein -
N0552_RS03175 (649417) 649417..649920 - 504 WP_001333820.1 M48 family metallopeptidase -
N0552_RS03180 (650005) 650005..651141 + 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
N0552_RS03185 (651425) 651425..651739 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
N0552_RS03190 (651736) 651736..652152 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
N0552_RS03195 (652197) 652197..654215 - 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
N0552_RS03200 (654641) 654641..656992 - 2352 WP_000695487.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T264294 WP_000550189.1 NZ_CP110814:651425-651739 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT264294 WP_000560266.1 NZ_CP110814:651736-652152 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References