Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3236089..3236737 Replicon chromosome
Accession NZ_CP110775
Organism Citrobacter freundii strain Cf-Emp

Toxin (Protein)


Gene name higB Uniprot ID A0A0D7M266
Locus tag OPR78_RS16140 Protein ID WP_016151211.1
Coordinates 3236375..3236737 (-) Length 121 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0D7LEH2
Locus tag OPR78_RS16135 Protein ID WP_003840660.1
Coordinates 3236089..3236388 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OPR78_RS16115 (3232411) 3232411..3232827 - 417 WP_003023951.1 GNAT family N-acetyltransferase -
OPR78_RS16120 (3232958) 3232958..3233251 + 294 WP_003840665.1 YicS family protein -
OPR78_RS16125 (3233346) 3233346..3234728 + 1383 WP_016151210.1 glycoside hydrolase family 1 protein -
OPR78_RS16130 (3234777) 3234777..3235970 - 1194 WP_003840662.1 purine ribonucleoside efflux pump NepI -
OPR78_RS16135 (3236089) 3236089..3236388 - 300 WP_003840660.1 XRE family transcriptional regulator Antitoxin
OPR78_RS16140 (3236375) 3236375..3236737 - 363 WP_016151211.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
OPR78_RS16145 (3236838) 3236838..3237290 - 453 WP_003023933.1 DUF1198 family protein -
OPR78_RS16150 (3237416) 3237416..3238807 - 1392 WP_003023929.1 hexose-6-phosphate:phosphate antiporter -
OPR78_RS16155 (3238950) 3238950..3240278 - 1329 WP_003023927.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(31-84)


Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13788.66 Da        Isoelectric Point: 5.1976

>T264208 WP_016151211.1 NZ_CP110775:c3236737-3236375 [Citrobacter freundii]
MWEVETTDAFDKWFDVQTEALKEDMLAAMMILSEYGPQLGRPFADTVNASAFSNMKELRVQHQGSPIRAFFAFDPSRHGI
VLCAGDKTGLNEKKFYKEMIRLADAEYRNHLISKENYGYP

Download         Length: 363 bp

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 11016.85 Da        Isoelectric Point: 8.5047

>AT264208 WP_003840660.1 NZ_CP110775:c3236388-3236089 [Citrobacter freundii]
MATLKELMAKQSPESQERITEKVEILRQAVALNMLREELNLSQAELASAMGVKQPTIAKMEQADNDPRLSTLKRYVTALG
GELSIDVKLPTGKRVAFYL

Download         Length: 300 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0D7M266

Antitoxin

Source ID Structure
AlphaFold DB A0A0D7LEH2

References