Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2268378..2269297 Replicon chromosome
Accession NZ_CP110619
Organism Mycobacterium tuberculosis strain H37Rv-1

Toxin (Protein)


Gene name HigB2 Uniprot ID L7N4R2
Locus tag M7V54_RS10690 Protein ID WP_003900449.1
Coordinates 2268692..2269297 (-) Length 202 a.a.

Antitoxin (Protein)


Gene name HigA2 Uniprot ID L7N5K9
Locus tag M7V54_RS10685 Protein ID WP_003410124.1
Coordinates 2268378..2268683 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M7V54_RS10655 2263389..2264645 - 1257 WP_003410095.1 HNH endonuclease signature motif containing protein -
M7V54_RS10660 2264999..2265574 + 576 WP_003410100.1 hypothetical protein -
M7V54_RS10665 2265571..2266611 + 1041 WP_003911735.1 ImmA/IrrE family metallo-endopeptidase -
M7V54_RS10670 2266853..2267572 + 720 WP_003410108.1 DUF433 domain-containing protein -
M7V54_RS10675 2267562..2267978 + 417 WP_003410114.1 hypothetical protein -
M7V54_RS10680 2267994..2268293 - 300 WP_003410120.1 hypothetical protein -
M7V54_RS10685 2268378..2268683 - 306 WP_003410124.1 XRE family transcriptional regulator Antitoxin
M7V54_RS10690 2268692..2269297 - 606 WP_003900449.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
M7V54_RS10695 2269322..2269681 - 360 WP_003410131.1 hypothetical protein -
M7V54_RS10700 2269841..2270236 - 396 WP_079156352.1 hypothetical protein -
M7V54_RS10705 2270296..2271813 - 1518 Protein_2116 DEAD/DEAH box helicase family protein -
M7V54_RS10710 2272323..2273321 - 999 WP_003410146.1 cation diffusion facilitator family transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(86-181)

Antitoxin

HigA2-like, helix-turn-helix domain

(32-92)


Sequences

Toxin        


Download         Length: 202 a.a.        Molecular weight: 22744.96 Da        Isoelectric Point: 7.3073

>T264004 WP_003900449.1 NZ_CP110619:c2269297-2268692 [Mycobacterium tuberculosis]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLVDTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Download         Length: 606 bp

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11291.91 Da        Isoelectric Point: 10.7482

>AT264004 WP_003410124.1 NZ_CP110619:c2268683-2268378 [Mycobacterium tuberculosis]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Download         Length: 306 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BV20

Antitoxin

Source ID Structure
PDB 7EWC
PDB 7EWD
PDB 7EWE
AlphaFold DB A0A7U4BV30

References