Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 258..910 Replicon plasmid pRWS292.s5
Accession NZ_CP110528
Organism Klebsiella pneumoniae strain WS_29-2

Toxin (Protein)


Gene name higB Uniprot ID B2CBE9
Locus tag OM422_RS30310 Protein ID WP_012457127.1
Coordinates 258..608 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID B2CBE8
Locus tag OM422_RS30315 Protein ID WP_012457126.1
Coordinates 611..910 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OM422_RS30310 (OM422_30295) 258..608 + 351 WP_012457127.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
OM422_RS30315 (OM422_30300) 611..910 + 300 WP_012457126.1 helix-turn-helix domain-containing protein Antitoxin
OM422_RS30320 (OM422_30305) 972..1121 - 150 WP_162262154.1 hypothetical protein -
OM422_RS30325 (OM422_30310) 1284..1949 - 666 WP_094309421.1 hypothetical protein -
OM422_RS30330 (OM422_30315) 2334..3302 + 969 WP_012457123.1 zincin-like metallopeptidase domain-containing protein -
OM422_RS30335 (OM422_30320) 3486..4016 - 531 WP_012457122.1 phospholipase D family protein -
OM422_RS30340 (OM422_30325) 4119..4874 - 756 WP_023316386.1 MobC family replication-relaxation protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..41699 41699


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(34-83)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13378.26 Da        Isoelectric Point: 6.7130

>T263918 WP_012457127.1 NZ_CP110528:258-608 [Klebsiella pneumoniae]
MWDIITTECFDGWFLAQSDALRESVYEAMGVLEKFGPALGRPYVDTLNDSDFANMKELRIQHHGNPVRAFFAFDPTRRAI
VLCAGDKTGANEKRFYKDMIRLADSEYRKHLAKLEK

Download         Length: 351 bp

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 11011.75 Da        Isoelectric Point: 5.8719

>AT263918 WP_012457126.1 NZ_CP110528:611-910 [Klebsiella pneumoniae]
MATYRELLAKESPEMQARVAERVEEVSIQIALSMLRDELNISQTELAAVMGVKQPSVARMEQADNDPRLSTLKRYVKALG
GELSLDVTLPTGKRVAFHI

Download         Length: 300 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB B2CBE9

Antitoxin

Source ID Structure
AlphaFold DB B2CBE8

References