Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 21278..21930 Replicon plasmid pRWS291.s5
Accession NZ_CP110519
Organism Klebsiella pneumoniae strain WS_29-1

Toxin (Protein)


Gene name higB Uniprot ID B2CBE9
Locus tag OM421_RS30405 Protein ID WP_012457127.1
Coordinates 21580..21930 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID B2CBE8
Locus tag OM421_RS30400 Protein ID WP_012457126.1
Coordinates 21278..21577 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OM421_RS30375 (OM421_30370) 17314..18069 + 756 WP_023316386.1 MobC family replication-relaxation protein -
OM421_RS30380 (OM421_30375) 18172..18702 + 531 WP_012457122.1 phospholipase D family protein -
OM421_RS30385 (OM421_30380) 18886..19854 - 969 WP_012457123.1 zincin-like metallopeptidase domain-containing protein -
OM421_RS30390 (OM421_30385) 20239..20904 + 666 WP_094309421.1 hypothetical protein -
OM421_RS30395 (OM421_30390) 21067..21216 + 150 WP_162262154.1 hypothetical protein -
OM421_RS30400 (OM421_30395) 21278..21577 - 300 WP_012457126.1 helix-turn-helix domain-containing protein Antitoxin
OM421_RS30405 (OM421_30400) 21580..21930 - 351 WP_012457127.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
OM421_RS30410 (OM421_30405) 22324..22941 + 618 WP_094309423.1 ParA family protein -
OM421_RS30415 (OM421_30410) 22996..23235 + 240 WP_012457129.1 plasmid partition protein ParG -
OM421_RS30420 (OM421_30415) 23398..25020 - 1623 WP_065813436.1 ABC-F family ATP-binding cassette domain-containing protein -
OM421_RS30425 (OM421_30420) 25445..26149 - 705 WP_001067855.1 IS6-like element IS26 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..38030 38030


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(34-83)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13378.26 Da        Isoelectric Point: 6.7130

>T263891 WP_012457127.1 NZ_CP110519:c21930-21580 [Klebsiella pneumoniae]
MWDIITTECFDGWFLAQSDALRESVYEAMGVLEKFGPALGRPYVDTLNDSDFANMKELRIQHHGNPVRAFFAFDPTRRAI
VLCAGDKTGANEKRFYKDMIRLADSEYRKHLAKLEK

Download         Length: 351 bp

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 11011.75 Da        Isoelectric Point: 5.8719

>AT263891 WP_012457126.1 NZ_CP110519:c21577-21278 [Klebsiella pneumoniae]
MATYRELLAKESPEMQARVAERVEEVSIQIALSMLRDELNISQTELAAVMGVKQPSVARMEQADNDPRLSTLKRYVKALG
GELSLDVTLPTGKRVAFHI

Download         Length: 300 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB B2CBE9

Antitoxin

Source ID Structure
AlphaFold DB B2CBE8

References