Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 1991887..1992428 Replicon chromosome
Accession NZ_CP110511
Organism Escherichia coli strain ws5-3

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag OM087_RS09660 Protein ID WP_000615976.1
Coordinates 1992150..1992428 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag OM087_RS09655 Protein ID WP_000729704.1
Coordinates 1991887..1992147 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OM087_RS09640 (1987844) 1987844..1989937 + 2094 WP_115724218.1 flagellar biosynthesis protein FlhA -
OM087_RS09645 (1990255) 1990255..1990752 - 498 WP_000006256.1 REP-associated tyrosine transposase RayT -
OM087_RS09650 (1990928) 1990928..1991701 - 774 WP_162822839.1 C40 family peptidase -
OM087_RS09655 (1991887) 1991887..1992147 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
OM087_RS09660 (1992150) 1992150..1992428 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
OM087_RS09665 (1992584) 1992584..1993324 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
OM087_RS09670 (1993295) 1993295..1994062 - 768 WP_000333380.1 class II glutamine amidotransferase -
OM087_RS09675 (1994268) 1994268..1994846 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA / rhs/PAAR / rhs/PAAR 1985949..2011381 25432


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T263847 WP_000615976.1 NZ_CP110511:1992150-1992428 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT263847 WP_000729704.1 NZ_CP110511:1991887-1992147 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References