Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 2845527..2846302 Replicon chromosome
Accession NZ_CP110345
Organism Pseudomonas aeruginosa strain PALA30

Toxin (Protein)


Gene name yhaV Uniprot ID V6ADY6
Locus tag PALA30_RS13655 Protein ID WP_009518525.1
Coordinates 2845844..2846302 (+) Length 153 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID V6AEP7
Locus tag PALA30_RS13650 Protein ID WP_023098543.1
Coordinates 2845527..2845844 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PALA30_RS13630 (PALA30_02693) 2840700..2842085 - 1386 Protein_2693 efflux RND transporter permease subunit -
PALA30_RS13635 (PALA30_02694) 2842222..2843133 + 912 WP_023098540.1 NAD(P)H-binding protein -
PALA30_RS13640 2843282..2843428 + 147 WP_023098541.1 MerR family DNA-binding transcriptional regulator -
PALA30_RS13645 (PALA30_02696) 2843410..2845227 - 1818 WP_023098542.1 MobH family relaxase -
PALA30_RS13650 (PALA30_02697) 2845527..2845844 + 318 WP_023098543.1 type II toxin-antitoxin system PrlF family antitoxin Antitoxin
PALA30_RS13655 (PALA30_02698) 2845844..2846302 + 459 WP_009518525.1 type II toxin-antitoxin system YhaV family toxin Toxin
PALA30_RS13660 (PALA30_02699) 2846329..2846706 + 378 WP_009518524.1 DUF3742 family protein -
PALA30_RS13665 (PALA30_02700) 2846722..2848239 - 1518 WP_009518523.1 conjugal transfer protein TraG N-terminal domain-containing protein -
PALA30_RS13670 (PALA30_02701) 2848254..2848613 - 360 WP_009518522.1 hypothetical protein -
PALA30_RS13675 (PALA30_02702) 2848610..2850004 - 1395 WP_009518521.1 integrating conjugative element protein -
PALA30_RS13680 (PALA30_02703) 2850014..2850961 - 948 WP_009518520.1 TIGR03756 family integrating conjugative element protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 2775080..2910396 135316


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(8-144)

Antitoxin

PrlF antitoxin

(8-100)


Sequences

Toxin        


Download         Length: 153 a.a.        Molecular weight: 17660.02 Da        Isoelectric Point: 10.1848

>T263637 WP_009518525.1 NZ_CP110345:2845844-2846302 [Pseudomonas aeruginosa]
MQRHGWTLLFHDCVIEQLQKLHAAARRAQENDPAGFESNANVKLFRALSQLMLDVVPGDPARDEYRQGNTLGPAHRHWRR
AKIGRRFRLFFRYDSKAKVIVYAWVNDEQTLRSSGSKSDPYVVFEKMLGRGNPPDDWHALIQASKQDWSKLE

Download         Length: 459 bp

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11170.72 Da        Isoelectric Point: 4.7182

>AT263637 WP_023098543.1 NZ_CP110345:2845527-2845844 [Pseudomonas aeruginosa]
MPAIHEVATLTSKGQITLPKPIRQALGVDAGGKLAFDLRGSEVVVTRVDAEHEDPAIGAFLSLLARDIEAGRNVRGLPED
LARAMLEHAGHGVNLDEEIDGKVEL

Download         Length: 318 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V6ADY6

Antitoxin

Source ID Structure
AlphaFold DB V6AEP7

References