Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 639217..639944 Replicon chromosome
Accession NZ_CP110258
Organism Escherichia albertii strain KBSW50i

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag N6N69_RS03210 Protein ID WP_000550189.1
Coordinates 639217..639531 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag N6N69_RS03215 Protein ID WP_105227497.1
Coordinates 639528..639944 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N6N69_RS03190 (635376) 635376..636362 - 987 WP_103053951.1 Gfo/Idh/MocA family oxidoreductase -
N6N69_RS03195 (636441) 636441..637133 - 693 WP_025238291.1 vancomycin high temperature exclusion protein -
N6N69_RS03200 (637210) 637210..637713 - 504 WP_044709508.1 M48 family metallopeptidase -
N6N69_RS03205 (637798) 637798..638934 + 1137 WP_059241776.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
N6N69_RS03210 (639217) 639217..639531 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
N6N69_RS03215 (639528) 639528..639944 + 417 WP_105227497.1 helix-turn-helix domain-containing protein Antitoxin
N6N69_RS03220 (640034) 640034..642052 - 2019 WP_103053949.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
N6N69_RS03225 (642495) 642495..644846 - 2352 WP_000695424.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T263480 WP_000550189.1 NZ_CP110258:639217-639531 [Escherichia albertii]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T263480 NZ_CP110258:639217-639531 [Escherichia albertii]
ATGCACCTGATCACTCAAAAAGCATTGAAAGATGCAGCGGAAAAATATCCGCAGCATAAAACGGAGTTGGTGGCACTGGG
GAACACGATTGCTAAGGGATACTTCAAAAAACCTGAGTCATTAAAAGCAGTGTTCCCATCTCTGGATAACTTTAAATATC
TGGATAAGCATTATGTTTTCAATGTCGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCACAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15047.49 Da        Isoelectric Point: 4.2698

>AT263480 WP_105227497.1 NZ_CP110258:639528-639944 [Escherichia albertii]
MIAIADILQVGEKLTDVAPFLAGIQSEEQYAQALELVDYLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMVQAMPGG
VAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLNGKRKLTLEHAKKLAARFGISPALFID

Download         Length: 417 bp

>AT263480 NZ_CP110258:639528-639944 [Escherichia albertii]
ATGATTGCGATTGCCGATATCTTGCAAGTAGGAGAAAAGCTAACCGATGTGGCACCTTTTCTGGCGGGTATCCAGAGCGA
AGAACAGTATGCCCAGGCGCTGGAACTGGTAGATTATCTGCTGCTCAACGATCCTGAAAATCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCTGAATTTAATGCGATGGTTCAAGCCATGCCTGGCGGT
GTTGCTGTGATTCGTACCCTGATGGATCAATACGGCTTAACCCTTTCCGATCTTCCAGAAATTGGCAGTAAATCCATGGT
GTCACGCGTTTTGAACGGGAAGAGAAAATTAACGCTGGAACACGCTAAAAAACTGGCAGCGCGATTCGGCATTTCCCCCG
CGTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References