Detailed information of TA system TA263180

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1126976..1127555
• Replicon: chromosome
• Accession: NZ_CP110130
• Organism: Methylorubrum extorquens strain NBC_00404

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: OKC48_RS05285
• Protein ID: WP_265222550.1
• Coordinates: 1126976..1127257 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A1W6RKV4
• Locus tag: OKC48_RS05290
• Protein ID: WP_056462529.1
• Coordinates: 1127271..1127555 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OKC48_RS05270 (OKC48_05270)	1122383..1122904	+	522	WP_003602674.1	translation initiation factor IF-3	-
OKC48_RS05275 (OKC48_05275)	1123096..1125432	-	2337	WP_056111703.1	glycogen debranching N-terminal domain-containing protein	-
OKC48_RS05280 (OKC48_05280)	1125591..1126724	-	1134	WP_056111700.1	glycosyltransferase family 4 protein	-
OKC48_RS05285 (OKC48_05285)	1126976..1127257	+	282	WP_265222550.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
OKC48_RS05290 (OKC48_05290)	1127271..1127555	+	285	WP_056462529.1	HigA family addiction module antitoxin	Antitoxin
OKC48_RS05295 (OKC48_05295)	1127749..1127952	+	204	WP_003602670.1	50S ribosomal protein L35	-
OKC48_RS05300 (OKC48_05300)	1128005..1128370	+	366	WP_003602669.1	50S ribosomal protein L20	-
OKC48_RS05305 (OKC48_05305)	1128554..1129633	+	1080	WP_015950513.1	phenylalanine--tRNA ligase subunit alpha	-
OKC48_RS05310 (OKC48_05310)	1129698..1132121	+	2424	WP_265245080.1	phenylalanine--tRNA ligase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (5-79)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10726.03 Da
• Isoelectric Point: 7.8895

>T263180 WP_265222550.1 NZ_CP110130:1126976-1127257 [Methylorubrum extorquens]
MILSYRNRGTEALDVHGVCHRRWRSFQAAAGRKLDMLNAASVVSDLRSPPGNRLEKLSGDREGQWSIRINDQWRICFRWD
GSGPEDVEIVDYH

Nucleotide

• Length: 282 bp

>T263180 NZ_CP110130:1126976-1127257 [Methylorubrum extorquens]
ATGATCCTCAGCTACCGCAACAGGGGCACCGAAGCGCTGGACGTGCACGGTGTGTGCCATCGCCGCTGGCGGTCCTTTCA
AGCTGCGGCCGGGCGCAAGCTCGATATGCTGAACGCCGCCTCCGTCGTGAGCGACCTGCGCAGCCCGCCCGGCAACCGTC
TGGAGAAGCTGTCGGGTGATCGCGAGGGCCAGTGGAGCATCCGCATCAACGATCAATGGCGCATCTGCTTCCGATGGGAC
GGTTCGGGGCCCGAGGATGTCGAGATTGTCGATTATCACTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10570.11 Da
• Isoelectric Point: 7.2787

>AT263180 WP_056462529.1 NZ_CP110130:1127271-1127555 [Methylorubrum extorquens]
MPRIRSHPGEILLEEYLRPLEMSANALAKAIEVPANRISELLRGRRGMTADTACRLGRFFGTTPEFWMSLQTAHDLSRAV
AEHDYSTLRALESA

Nucleotide

• Length: 285 bp

>AT263180 NZ_CP110130:1127271-1127555 [Methylorubrum extorquens]
ATGCCTCGCATTAGATCTCATCCGGGCGAGATCCTGCTCGAAGAATACCTTCGACCCCTGGAAATGAGCGCCAACGCTCT
GGCGAAGGCGATCGAGGTGCCAGCCAACCGCATCAGCGAACTTCTGCGCGGTCGCCGCGGGATGACCGCCGACACGGCCT
GCCGCCTCGGCCGGTTCTTCGGGACCACGCCGGAATTCTGGATGAGTCTCCAGACCGCCCACGATCTGTCGAGGGCCGTC
GCGGAGCACGACTATTCCACCTTGCGTGCTCTCGAAAGCGCCTGA

Structures

Antitoxin

• AlphaFold DB: A0A1W6RKV4