Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3885009..3885703 Replicon chromosome
Accession NZ_CP110117
Organism Escherichia coli strain 2-331

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag OMD12_RS19055 Protein ID WP_001263489.1
Coordinates 3885009..3885407 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag OMD12_RS19060 Protein ID WP_000554758.1
Coordinates 3885410..3885703 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (3880597) 3880597..3880677 - 81 NuclAT_11 - -
- (3880597) 3880597..3880677 - 81 NuclAT_11 - -
- (3880597) 3880597..3880677 - 81 NuclAT_11 - -
- (3880597) 3880597..3880677 - 81 NuclAT_11 - -
OMD12_RS19030 (3881273) 3881273..3881731 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
OMD12_RS19035 (3881992) 3881992..3883449 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
OMD12_RS19040 (3883506) 3883506..3884027 - 522 Protein_3730 peptide chain release factor H -
OMD12_RS19045 (3884023) 3884023..3884229 - 207 Protein_3731 RtcB family protein -
OMD12_RS19050 (3884547) 3884547..3884999 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
OMD12_RS19055 (3885009) 3885009..3885407 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
OMD12_RS19060 (3885410) 3885410..3885703 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
OMD12_RS19065 (3885755) 3885755..3886810 - 1056 WP_001226164.1 DNA polymerase IV -
OMD12_RS19070 (3886881) 3886881..3887666 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
OMD12_RS19075 (3887638) 3887638..3889350 + 1713 Protein_3737 flagellar biosynthesis protein FlhA -
OMD12_RS19080 (3889574) 3889574..3890071 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 3885009..3900289 15280


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T263142 WP_001263489.1 NZ_CP110117:c3885407-3885009 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT263142 WP_000554758.1 NZ_CP110117:c3885703-3885410 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References