Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 226109..226803 Replicon chromosome
Accession NZ_CP110017
Organism Escherichia coli strain HE_50

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag OLR76_RS01080 Protein ID WP_001263489.1
Coordinates 226405..226803 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag OLR76_RS01075 Protein ID WP_000554758.1
Coordinates 226109..226402 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OLR76_RS01055 (221742) 221742..222239 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
OLR76_RS01060 (222463) 222463..224175 - 1713 Protein_204 flagellar biosynthesis protein FlhA -
OLR76_RS01065 (224147) 224147..224932 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
OLR76_RS01070 (225003) 225003..226057 + 1055 Protein_206 DNA polymerase IV -
OLR76_RS01075 (226109) 226109..226402 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
OLR76_RS01080 (226405) 226405..226803 + 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
OLR76_RS01085 (226813) 226813..227265 + 453 WP_001059892.1 GNAT family N-acetyltransferase -
OLR76_RS01090 (227583) 227583..227789 + 207 Protein_210 RtcB family protein -
OLR76_RS01095 (227785) 227785..228306 + 522 Protein_211 peptide chain release factor H -
OLR76_RS01100 (228363) 228363..229820 - 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
OLR76_RS01105 (230081) 230081..230539 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- (231135) 231135..231215 + 81 NuclAT_12 - -
- (231135) 231135..231215 + 81 NuclAT_12 - -
- (231135) 231135..231215 + 81 NuclAT_12 - -
- (231135) 231135..231215 + 81 NuclAT_12 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T262711 WP_001263489.1 NZ_CP110017:226405-226803 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT262711 WP_000554758.1 NZ_CP110017:226109-226402 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References