Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3330838..3331565 Replicon chromosome
Accession NZ_CP110016
Organism Escherichia coli strain HE_100

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag OLR74_RS16400 Protein ID WP_000550189.1
Coordinates 3331251..3331565 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag OLR74_RS16395 Protein ID WP_000560266.1
Coordinates 3330838..3331254 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OLR74_RS16385 (3325998) 3325998..3328349 + 2352 WP_000695487.1 alpha-glucosidase -
OLR74_RS16390 (3328775) 3328775..3330793 + 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
OLR74_RS16395 (3330838) 3330838..3331254 - 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
OLR74_RS16400 (3331251) 3331251..3331565 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
OLR74_RS16405 (3331849) 3331849..3332985 - 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
OLR74_RS16410 (3333070) 3333070..3333573 + 504 WP_001333820.1 M48 family metallopeptidase -
OLR74_RS16415 (3333650) 3333650..3334342 + 693 WP_000942548.1 vancomycin high temperature exclusion protein -
OLR74_RS16420 (3334421) 3334421..3335407 + 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T262705 WP_000550189.1 NZ_CP110016:c3331565-3331251 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT262705 WP_000560266.1 NZ_CP110016:c3331254-3330838 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References