Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/HigB-HigA |
| Location | 32624..33351 | Replicon | plasmid pA |
| Accession | NZ_CP109974 | ||
| Organism | Klebsiella pneumoniae strain 857 | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | A0A7W3D9W1 |
| Locus tag | OI501_RS27030 | Protein ID | WP_011251285.1 |
| Coordinates | 32624..32935 (+) | Length | 104 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | - |
| Locus tag | OI501_RS27035 | Protein ID | WP_011251286.1 |
| Coordinates | 32932..33351 (+) | Length | 140 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| OI501_RS27005 (OI501_27010) | 28143..28637 | - | 495 | WP_004212794.1 | thermonuclease family protein | - |
| OI501_RS27010 (OI501_27015) | 28815..29081 | + | 267 | WP_223175074.1 | DUF1173 family protein | - |
| OI501_RS27015 (OI501_27020) | 29114..29764 | + | 651 | WP_068893702.1 | DUF1173 family protein | - |
| OI501_RS27025 (OI501_27030) | 31982..32419 | + | 438 | Protein_35 | DDE-type integrase/transposase/recombinase | - |
| OI501_RS27030 (OI501_27035) | 32624..32935 | + | 312 | WP_011251285.1 | type II toxin-antitoxin system HigB family toxin | Toxin |
| OI501_RS27035 (OI501_27040) | 32932..33351 | + | 420 | WP_011251286.1 | helix-turn-helix domain-containing protein | Antitoxin |
| OI501_RS27040 (OI501_27045) | 33498..34466 | + | 969 | WP_011251287.1 | IS5 family transposase | - |
| OI501_RS27045 (OI501_27050) | 34538..34903 | - | 366 | WP_048333448.1 | hypothetical protein | - |
| OI501_RS27050 (OI501_27055) | 34917..35705 | - | 789 | WP_040217257.1 | hypothetical protein | - |
| OI501_RS27055 (OI501_27060) | 35726..36346 | - | 621 | WP_223175004.1 | conjugative transfer protein MobI(A/C) | - |
| OI501_RS27060 (OI501_27065) | 36765..37400 | + | 636 | WP_223171879.1 | hypothetical protein | - |
| OI501_RS27065 (OI501_27070) | 37713..38183 | + | 471 | WP_048333570.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Mobilizable plasmid | - | iroN / iucA / iucB / iucC / iucD / iutA / rmpA2 | 1..192941 | 192941 | |
| - | inside | IScluster/Tn | - | - | 16168..41191 | 25023 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 104 a.a. Molecular weight: 12346.12 Da Isoelectric Point: 9.7248
>T262593 WP_011251285.1 NZ_CP109974:32624-32935 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE
Download Length: 312 bp
Antitoxin
Download Length: 140 a.a. Molecular weight: 15377.46 Da Isoelectric Point: 4.4420
>AT262593 WP_011251286.1 NZ_CP109974:32932-33351 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL
Download Length: 420 bp
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|