Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4789156..4789883 Replicon chromosome
Accession NZ_CP109953
Organism Escherichia coli strain NC22

Toxin (Protein)


Gene name higB Uniprot ID J7Q991
Locus tag OI124_RS23305 Protein ID WP_000547564.1
Coordinates 4789156..4789467 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag OI124_RS23310 Protein ID WP_000126294.1
Coordinates 4789464..4789883 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OI124_RS23275 (4784298) 4784298..4786007 + 1710 WP_001288134.1 formate hydrogenlyase subunit HycE -
OI124_RS23280 (4786017) 4786017..4786559 + 543 WP_000493792.1 formate hydrogenlyase subunit HycF -
OI124_RS23285 (4786559) 4786559..4787326 + 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
OI124_RS23290 (4787323) 4787323..4787733 + 411 WP_228569532.1 formate hydrogenlyase assembly protein HycH -
OI124_RS23295 (4787726) 4787726..4788196 + 471 WP_000132961.1 hydrogenase maturation peptidase HycI -
OI124_RS23300 (4788221) 4788221..4788982 + 762 WP_001026446.1 hypothetical protein -
OI124_RS23305 (4789156) 4789156..4789467 + 312 WP_000547564.1 type II toxin-antitoxin system HigB family toxin Toxin
OI124_RS23310 (4789464) 4789464..4789883 + 420 WP_000126294.1 helix-turn-helix domain-containing protein Antitoxin
OI124_RS23315 (4789997) 4789997..4791421 - 1425 WP_000110363.1 6-phospho-beta-glucosidase AscB -
OI124_RS23320 (4791430) 4791430..4792887 - 1458 WP_001107861.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
OI124_RS23325 (4793147) 4793147..4794157 + 1011 WP_001392554.1 DNA-binding transcriptional regulator AscG -
OI124_RS23330 (4794306) 4794306..4794833 + 528 WP_001078777.1 electron transport protein HydN -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12426.13 Da        Isoelectric Point: 9.7248

>T262536 WP_000547564.1 NZ_CP109953:4789156-4789467 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15416.42 Da        Isoelectric Point: 4.4596

>AT262536 WP_000126294.1 NZ_CP109953:4789464-4789883 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FJG5

Antitoxin

Source ID Structure

References