Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4224975..4225774 Replicon chromosome
Accession NZ_CP109953
Organism Escherichia coli strain NC22

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag OI124_RS20475 Protein ID WP_000347251.1
Coordinates 4224975..4225439 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag OI124_RS20480 Protein ID WP_001307405.1
Coordinates 4225439..4225774 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OI124_RS20445 (4219976) 4219976..4220410 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
OI124_RS20450 (4220428) 4220428..4221306 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
OI124_RS20455 (4221296) 4221296..4222075 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
OI124_RS20460 (4222086) 4222086..4222559 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
OI124_RS20465 (4222582) 4222582..4223862 - 1281 WP_000681903.1 tagatose-bisphosphate aldolase subunit KbaZ -
OI124_RS20470 (4224111) 4224111..4224920 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
OI124_RS20475 (4224975) 4224975..4225439 - 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
OI124_RS20480 (4225439) 4225439..4225774 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
OI124_RS20485 (4225923) 4225923..4227494 - 1572 WP_048943282.1 galactarate dehydratase -
OI124_RS20490 (4227869) 4227869..4229203 + 1335 WP_000599652.1 galactarate/glucarate/glycerate transporter GarP -
OI124_RS20495 (4229219) 4229219..4229989 + 771 WP_001058214.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T262532 WP_000347251.1 NZ_CP109953:c4225439-4224975 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT262532 WP_001307405.1 NZ_CP109953:c4225774-4225439 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References