Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-VapI
Location 52450..53045 Replicon chromosome
Accession NZ_CP109932
Organism Pseudomonas aeruginosa strain PALA43

Toxin (Protein)


Gene name higB Uniprot ID V6ALY3
Locus tag PALA43_RS00275 Protein ID WP_003113526.1
Coordinates 52767..53045 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag PALA43_RS00270 Protein ID WP_003113527.1
Coordinates 52450..52755 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PALA43_RS00235 (PALA43_00047) 47589..48437 + 849 WP_003099284.1 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase -
PALA43_RS00245 (PALA43_00049) 48604..49545 + 942 WP_269972417.1 ribose-phosphate pyrophosphokinase -
PALA43_RS00250 (PALA43_00050) 49662..50276 + 615 WP_003099279.1 50S ribosomal protein L25/general stress protein Ctc -
PALA43_RS00255 (PALA43_00051) 50318..50902 + 585 WP_003099278.1 aminoacyl-tRNA hydrolase -
PALA43_RS00260 (PALA43_00052) 50943..52043 + 1101 WP_003099270.1 redox-regulated ATPase YchF -
PALA43_RS00270 (PALA43_00054) 52450..52755 - 306 WP_003113527.1 HigA family addiction module antitoxin Antitoxin
PALA43_RS00275 52767..53045 - 279 WP_003113526.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
PALA43_RS00280 53098..53226 - 129 Protein_53 integrase -
PALA43_RS00285 (PALA43_00055) 53374..55602 + 2229 WP_003113525.1 TonB-dependent receptor -
PALA43_RS00290 (PALA43_00056) 55672..56319 - 648 WP_003095021.1 carbonate dehydratase -
PALA43_RS00295 (PALA43_00057) 56381..57619 - 1239 WP_003113524.1 dipeptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(5-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(22-69)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10646.19 Da        Isoelectric Point: 7.8937

>T262425 WP_003113526.1 NZ_CP109932:c53045-52767 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Download         Length: 279 bp

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11247.81 Da        Isoelectric Point: 5.0747

>AT262425 WP_003113527.1 NZ_CP109932:c52755-52450 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Download         Length: 306 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V6ALY3

Antitoxin

Source ID Structure

References