Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2604161..2604723 Replicon chromosome
Accession NZ_CP109929
Organism Escherichia coli O6:K2:H1 strain C 691-04A

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag N8A71_RS12400 Protein ID WP_000605675.1
Coordinates 2604161..2604439 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag N8A71_RS12405 Protein ID WP_000781370.1
Coordinates 2604439..2604723 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N8A71_RS12375 (2599747) 2599747..2600178 - 432 WP_000152307.1 peroxiredoxin OsmC -
N8A71_RS12380 (2600523) 2600523..2600738 + 216 WP_000495766.1 biofilm-dependent modulation protein -
N8A71_RS12385 (2600840) 2600840..2600977 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
N8A71_RS12390 (2601134) 2601134..2602831 + 1698 WP_000433460.1 malate dehydrogenase -
N8A71_RS12395 (2602965) 2602965..2603975 + 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
N8A71_RS12400 (2604161) 2604161..2604439 + 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
N8A71_RS12405 (2604439) 2604439..2604723 + 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
N8A71_RS12410 (2604952) 2604952..2605605 - 654 WP_000045646.1 formate dehydrogenase-N subunit gamma -
N8A71_RS12415 (2605598) 2605598..2606482 - 885 WP_001240584.1 formate dehydrogenase N subunit beta -
N8A71_RS12420 (2606495) 2606495..2609542 - 3048 WP_011076399.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T262405 WP_000605675.1 NZ_CP109929:2604161-2604439 [Escherichia coli O6:K2:H1]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT262405 WP_000781370.1 NZ_CP109929:2604439-2604723 [Escherichia coli O6:K2:H1]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References