Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 622723..623522 Replicon chromosome
Accession NZ_CP109929
Organism Escherichia coli O6:K2:H1 strain C 691-04A

Toxin (Protein)


Gene name yhaV Uniprot ID Q8FDB4
Locus tag N8A71_RS03060 Protein ID WP_000347252.1
Coordinates 622723..623187 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1PPV5
Locus tag N8A71_RS03065 Protein ID WP_001296435.1
Coordinates 623187..623522 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N8A71_RS03030 (617724) 617724..618158 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
N8A71_RS03035 (618176) 618176..619054 - 879 WP_001298314.1 PTS N-acetylgalactosamine transporter subunit IID -
N8A71_RS03040 (619044) 619044..619823 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
N8A71_RS03045 (619834) 619834..620307 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
N8A71_RS03050 (620330) 620330..621610 - 1281 WP_000681926.1 tagatose-bisphosphate aldolase subunit KbaZ -
N8A71_RS03055 (621859) 621859..622668 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
N8A71_RS03060 (622723) 622723..623187 - 465 WP_000347252.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
N8A71_RS03065 (623187) 623187..623522 - 336 WP_001296435.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
N8A71_RS03070 (623671) 623671..625242 - 1572 WP_001273940.1 galactarate dehydratase -
N8A71_RS03075 (625617) 625617..626951 + 1335 WP_000979025.1 galactarate/glucarate/glycerate transporter GarP -
N8A71_RS03080 (626967) 626967..627737 + 771 WP_001058223.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17750.11 Da        Isoelectric Point: 9.4947

>T262394 WP_000347252.1 NZ_CP109929:c623187-622723 [Escherichia coli O6:K2:H1]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEESH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12321.92 Da        Isoelectric Point: 4.5392

>AT262394 WP_001296435.1 NZ_CP109929:c623522-623187 [Escherichia coli O6:K2:H1]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q8FDB4

Antitoxin

Source ID Structure
AlphaFold DB S1PPV5

References