Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1259108..1259835 Replicon chromosome
Accession NZ_CP109921
Organism Escherichia coli O2:K2:H1 strain C 237-04

Toxin (Protein)


Gene name higB Uniprot ID V0YLE2
Locus tag N7L43_RS06100 Protein ID WP_000547555.1
Coordinates 1259108..1259419 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0H2V9Z2
Locus tag N7L43_RS06105 Protein ID WP_000126293.1
Coordinates 1259416..1259835 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N7L43_RS06070 (1254249) 1254249..1255958 + 1710 WP_001288134.1 formate hydrogenlyase subunit HycE -
N7L43_RS06075 (1255968) 1255968..1256510 + 543 WP_000493797.1 formate hydrogenlyase subunit HycF -
N7L43_RS06080 (1256510) 1256510..1257277 + 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
N7L43_RS06085 (1257274) 1257274..1257684 + 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
N7L43_RS06090 (1257677) 1257677..1258147 + 471 WP_000132956.1 hydrogenase maturation peptidase HycI -
N7L43_RS06095 (1258190) 1258190..1258945 + 756 WP_001305268.1 hypothetical protein -
N7L43_RS06100 (1259108) 1259108..1259419 + 312 WP_000547555.1 type II toxin-antitoxin system HigB family toxin Toxin
N7L43_RS06105 (1259416) 1259416..1259835 + 420 WP_000126293.1 helix-turn-helix domain-containing protein Antitoxin
N7L43_RS06110 (1259927) 1259927..1260355 - 429 WP_171854703.1 hypothetical protein -
N7L43_RS06115 (1260740) 1260740..1261267 + 528 WP_001078782.1 electron transport protein HydN -
N7L43_RS06120 (1261420) 1261420..1263672 + 2253 WP_001107659.1 carbamoyltransferase HypF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12439.18 Da        Isoelectric Point: 9.5146

>T262354 WP_000547555.1 NZ_CP109921:1259108-1259419 [Escherichia coli O2:K2:H1]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15426.46 Da        Isoelectric Point: 4.4596

>AT262354 WP_000126293.1 NZ_CP109921:1259416-1259835 [Escherichia coli O2:K2:H1]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTIPHIKALSARFGVKPEWFL

Download         Length: 420 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0YLE2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H2V9Z2

References