262255

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3936455..3937149 Replicon chromosome
Accession NZ_CP109874
Organism Escherichia coli strain EC488

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag NQ150_RS19025 Protein ID WP_001263491.1
Coordinates 3936455..3936853 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag NQ150_RS19030 Protein ID WP_000554755.1
Coordinates 3936856..3937149 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NQ150_RS18995 (3931717) 3931717..3933174 + 1458 WP_022645227.1 cytosol nonspecific dipeptidase -
NQ150_RS19000 (3933183) 3933183..3933464 + 282 WP_022645226.1 hypothetical protein -
NQ150_RS19005 (3933481) 3933481..3933990 - 510 WP_001361775.1 metal-dependent hydrolase -
NQ150_RS19010 (3934052) 3934052..3934666 - 615 WP_022645225.1 peptide chain release factor H -
NQ150_RS19015 (3934663) 3934663..3935802 - 1140 WP_022645224.1 RNA ligase RtcB family protein -
NQ150_RS19020 (3935993) 3935993..3936445 - 453 WP_261630064.1 GNAT family N-acetyltransferase -
NQ150_RS19025 (3936455) 3936455..3936853 - 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
NQ150_RS19030 (3936856) 3936856..3937149 - 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
NQ150_RS19035 (3937201) 3937201..3938256 - 1056 WP_022645222.1 DNA polymerase IV -
NQ150_RS19040 (3938327) 3938327..3939112 - 786 WP_072224360.1 putative lateral flagellar export/assembly protein LafU -
NQ150_RS19045 (3939084) 3939084..3940796 + 1713 Protein_3732 flagellar biosynthesis protein FlhA -
NQ150_RS19050 (3940894) 3940894..3941667 - 774 WP_022645219.1 C40 family peptidase -
NQ150_RS19055 (3941853) 3941853..3942113 + 261 WP_000729708.1 type II toxin-antitoxin system antitoxin DinJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T262255 WP_001263491.1 NZ_CP109874:c3936853-3936455 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT262255 WP_000554755.1 NZ_CP109874:c3937149-3936856 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References