Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2460686..2461346 Replicon chromosome
Accession NZ_CP109694
Organism Enterobacter asburiae strain 2017-45-54-05

Toxin (Protein)


Gene name higB Uniprot ID A0A808IFD9
Locus tag OI909_RS12150 Protein ID WP_048980127.1
Coordinates 2460993..2461346 (-) Length 118 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A1Z3MX79
Locus tag OI909_RS12145 Protein ID WP_029741460.1
Coordinates 2460686..2460988 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OI909_RS12115 (OI909_12110) 2455793..2457982 + 2190 WP_047061141.1 TonB-dependent siderophore receptor -
OI909_RS12125 (OI909_12120) 2458277..2458609 - 333 WP_058841450.1 type II toxin-antitoxin system PemK/MazF family toxin -
OI909_RS12130 (OI909_12125) 2458610..2458867 - 258 WP_058841451.1 hypothetical protein -
OI909_RS12135 (OI909_12130) 2459159..2459548 - 390 WP_096150847.1 RidA family protein -
OI909_RS12140 (OI909_12135) 2459690..2460610 + 921 WP_048980124.1 LysR family transcriptional regulator -
OI909_RS12145 (OI909_12140) 2460686..2460988 - 303 WP_029741460.1 XRE family transcriptional regulator Antitoxin
OI909_RS12150 (OI909_12145) 2460993..2461346 - 354 WP_048980127.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
OI909_RS12155 (OI909_12150) 2461524..2462417 - 894 WP_048980130.1 LysR substrate-binding domain-containing protein -
OI909_RS12165 (OI909_12160) 2463927..2464532 + 606 WP_048980132.1 glutathione S-transferase family protein -
OI909_RS12170 (OI909_12165) 2464576..2465526 - 951 WP_023336219.1 HTH-type transcriptional regulator Cbl -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2458610..2474391 15781


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-106)

Antitoxin

Cro/C1-type helix-turn-helix domain

(33-79)


Sequences

Toxin        


Download         Length: 118 a.a.        Molecular weight: 13599.44 Da        Isoelectric Point: 8.9620

>T262004 WP_048980127.1 NZ_CP109694:c2461346-2460993 [Enterobacter asburiae]
VWAINTTDRFDRWFTSLDDTDRASVLAALLVLREKGPGLSRPYADTLKGSRYSNMKELRIQSKGDPLRAFFAFDPNRTGI
VLCAGNKVGNERRFYDEMLLVADREFTHWLDRLKERE

Download         Length: 354 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11050.83 Da        Isoelectric Point: 5.7316

>AT262004 WP_029741460.1 NZ_CP109694:c2460988-2460686 [Enterobacter asburiae]
MGRTLEQLIADEKPEVVASAQAIATDILLNIHLAELREKVQKTQVEMAQALGIRQPTVAVMEKPGRDLKLSSLKRYVEAA
GGKLRLDVELPDGSHYEFVL

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A808IFD9

Antitoxin

Source ID Structure
AlphaFold DB A0A1Z3MX79

References