Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3710700..3711394 Replicon chromosome
Accession NZ_CP109601
Organism Escherichia coli strain TL-14

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag OGV15_RS18135 Protein ID WP_001263489.1
Coordinates 3710700..3711098 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag OGV15_RS18140 Protein ID WP_000554758.1
Coordinates 3711101..3711394 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (3706288) 3706288..3706368 - 81 NuclAT_11 - -
- (3706288) 3706288..3706368 - 81 NuclAT_11 - -
- (3706288) 3706288..3706368 - 81 NuclAT_11 - -
- (3706288) 3706288..3706368 - 81 NuclAT_11 - -
OGV15_RS18110 (3706964) 3706964..3707422 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
OGV15_RS18115 (3707683) 3707683..3709140 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
OGV15_RS18120 (3709197) 3709197..3709718 - 522 Protein_3544 peptide chain release factor H -
OGV15_RS18125 (3709714) 3709714..3709920 - 207 Protein_3545 RtcB family protein -
OGV15_RS18130 (3710238) 3710238..3710690 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
OGV15_RS18135 (3710700) 3710700..3711098 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
OGV15_RS18140 (3711101) 3711101..3711394 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
OGV15_RS18145 (3711446) 3711446..3712501 - 1056 WP_001226164.1 DNA polymerase IV -
OGV15_RS18150 (3712572) 3712572..3713357 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
OGV15_RS18155 (3713329) 3713329..3715041 + 1713 Protein_3551 flagellar biosynthesis protein FlhA -
OGV15_RS18160 (3715265) 3715265..3715762 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T261862 WP_001263489.1 NZ_CP109601:c3711098-3710700 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT261862 WP_000554758.1 NZ_CP109601:c3711394-3711101 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References