Detailed information of TA system TA261720

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 2742891..2744342
• Replicon: chromosome
• Accession: NZ_CP107532
• Organism: Methylobacterium sp. FF17

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: OF380_RS12885
• Protein ID: WP_056355047.1
• Coordinates: 2742891..2743229 (+)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A1I2UXZ8
• Locus tag: OF380_RS12890
• Protein ID: WP_056355050.1
• Coordinates: 2743248..2744342 (+)
• Length: 365 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OF380_RS12865	2740279..2740755	+	477	WP_056355038.1	DUF411 domain-containing protein	-
OF380_RS12870	2740835..2741605	+	771	Protein_2556	cobalamin-binding protein	-
OF380_RS12875	2741930..2742271	+	342	WP_056355041.1	hypothetical protein	-
OF380_RS12880	2742338..2742601	-	264	WP_082487871.1	hypothetical protein	-
OF380_RS12885	2742891..2743229	+	339	WP_056355047.1	system killer suppression protein	Toxin
OF380_RS12890	2743248..2744342	+	1095	WP_056355050.1	HigA family addiction module antitoxin	Antitoxin
OF380_RS12895	2744403..2745473	-	1071	WP_056355301.1	murein L,D-transpeptidase family protein	-
OF380_RS12900	2745562..2746080	-	519	WP_177232401.1	signal peptidase II	-
OF380_RS12905	2746077..2748242	-	2166	WP_056355056.1	heavy metal translocating P-type ATPase	-
OF380_RS12910	2748360..2748761	+	402	WP_056355059.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-68)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 12454.26 Da
• Isoelectric Point: 8.5257

>T261720 WP_056355047.1 NZ_CP107532:2742891-2743229 [Methylobacterium sp. FF17]
MQIRFRSRRLGKALSDPKEMAKAYGDRAKPIRKRLDVLGQAACLSDVPTEPPVRRHQLGGNRDGQLSVTIKDNWRIVFVP
DHDPVPCLPDGGLDTGQVTAVEIIEIVDYHGN

Nucleotide

• Length: 339 bp

Antitoxin

Protein

• Length: 365 a.a.
• Molecular weight: 41078.89 Da
• Isoelectric Point: 6.5014

>AT261720 WP_056355050.1 NZ_CP107532:2743248-2744342 [Methylobacterium sp. FF17]
MTTQEAWTPQWATHPGEHLEEYIQERGWSQAEFARIADMTPKLVSTIINGSNPVTPETALKLEHVLGLKAYIWTGLQANW
DLFQARQREQEAAVREAEWLKGFPIRELKACGRLPQTNDPGRIMDGLLGLLGIGGPHAYEAKLGSLAVHHRKARTGTTSP
HHMLCWLMLGEEKARRLSLPAYDPGRFKQGCAEIRSLTVEMPETFEPRMLEICHASGVALVFQKPLSKTCVFGSARWLND
DRPMIQMSLRMKSNDHFWWTFFHEAAHISLHRGMNFVDDKNGEDNDVEAEADAWAEEMLVGRERIDAFATTCPRTKQEVV
TFADEVGLHPGIVVGMLQHRRVLAFSHLNDLKARLGWADEVAAA

Nucleotide

• Length: 1095 bp

Structures

Antitoxin

• AlphaFold DB: A0A1I2UXZ8