Detailed information of TA system TA261446

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 36502..36928
• Replicon: plasmid unnamed2
• Accession: NZ_CP107470
• Organism: Klebsiella pneumoniae strain CRKP_11

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: OH523_RS28280
• Protein ID: WP_001372321.1
• Coordinates: 36502..36627 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	36704..36928 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OH523_RS28245 (31557)	31557..31784	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
OH523_RS28250 (31878)	31878..32564	-	687	WP_015059009.1	PAS domain-containing protein	-
OH523_RS28255 (32755)	32755..33138	-	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
OH523_RS28260 (33415)	33415..34062	+	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
OH523_RS28265 (34359)	34359..35180	-	822	WP_001234445.1	DUF932 domain-containing protein	-
OH523_RS28270 (35291)	35291..35587	-	297	WP_001272251.1	hypothetical protein	-
OH523_RS28275 (35887)	35887..36183	+	297	Protein_40	hypothetical protein	-
OH523_RS28280 (36502)	36502..36627	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
OH523_RS28285 (36569)	36569..36718	-	150	Protein_42	plasmid maintenance protein Mok	-
- (36704)	36704..36928	-	225	NuclAT_0	-	Antitoxin
- (36704)	36704..36928	-	225	NuclAT_0	-	Antitoxin
- (36704)	36704..36928	-	225	NuclAT_0	-	Antitoxin
- (36704)	36704..36928	-	225	NuclAT_0	-	Antitoxin
OH523_RS28290 (36940)	36940..37659	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
OH523_RS28295 (37656)	37656..38090	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
OH523_RS28300 (38159)	38159..40182	-	2024	Protein_45	ParB/RepB/Spo0J family partition protein	-
OH523_RS28305 (40243)	40243..40476	-	234	WP_000006003.1	DUF905 family protein	-
OH523_RS28310 (40534)	40534..41061	-	528	WP_000290834.1	single-stranded DNA-binding protein	-
OH523_RS28315 (41363)	41363..41824	+	462	Protein_48	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	rmtB / blaTEM-1B / blaCTX-M-65 / blaKPC-2	-	1..154719	154719

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T261446 WP_001372321.1 NZ_CP107470:c36627-36502 [Klebsiella pneumoniae]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

Antitoxin

Nucleotide

• Length: 225 bp

>AT261446 NZ_CP107470:c36928-36704 [Klebsiella pneumoniae]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin