Detailed information of TA system TA261146

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 52412..52838
• Replicon: plasmid unnamed1
• Accession: NZ_CP107409
• Organism: Klebsiella pneumoniae strain CRKP_24

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: OH533_RS27775
• Protein ID: WP_001372321.1
• Coordinates: 52412..52537 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	52614..52838 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OH533_RS27740 (47467)	47467..47694	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
OH533_RS27745 (47788)	47788..48474	-	687	WP_015059009.1	PAS domain-containing protein	-
OH533_RS27750 (48665)	48665..49048	-	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
OH533_RS27755 (49325)	49325..49972	+	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
OH533_RS27760 (50269)	50269..51090	-	822	WP_001234445.1	DUF932 domain-containing protein	-
OH533_RS27765 (51201)	51201..51497	-	297	WP_001272251.1	hypothetical protein	-
OH533_RS27770 (51797)	51797..52093	+	297	Protein_59	hypothetical protein	-
OH533_RS27775 (52412)	52412..52537	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
OH533_RS27780 (52479)	52479..52628	-	150	Protein_61	plasmid maintenance protein Mok	-
- (52614)	52614..52838	-	225	NuclAT_0	-	Antitoxin
- (52614)	52614..52838	-	225	NuclAT_0	-	Antitoxin
- (52614)	52614..52838	-	225	NuclAT_0	-	Antitoxin
- (52614)	52614..52838	-	225	NuclAT_0	-	Antitoxin
OH533_RS27785 (52850)	52850..53569	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
OH533_RS27790 (53566)	53566..54000	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
OH533_RS27795 (54069)	54069..56092	-	2024	Protein_64	ParB/RepB/Spo0J family partition protein	-
OH533_RS27800 (56153)	56153..56386	-	234	WP_000006003.1	DUF905 family protein	-
OH533_RS27805 (56444)	56444..56971	-	528	WP_000290834.1	single-stranded DNA-binding protein	-
OH533_RS27810 (57273)	57273..57734	+	462	Protein_67	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaKPC-2 / blaCTX-M-65 / catA2	-	1..157273	157273

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T261146 WP_001372321.1 NZ_CP107409:c52537-52412 [Klebsiella pneumoniae]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

Antitoxin

Nucleotide

• Length: 225 bp

>AT261146 NZ_CP107409:c52838-52614 [Klebsiella pneumoniae]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin