Detailed information of TA system TA261003

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 37002..37428
• Replicon: plasmid unnamed1
• Accession: NZ_CP107380
• Organism: Klebsiella pneumoniae strain CRKP_30

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: OH532_RS27135
• Protein ID: WP_001372321.1
• Coordinates: 37002..37127 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	37204..37428 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OH532_RS27100 (32057)	32057..32284	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
OH532_RS27105 (32378)	32378..33064	-	687	WP_015059009.1	PAS domain-containing protein	-
OH532_RS27110 (33255)	33255..33638	-	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
OH532_RS27115 (33915)	33915..34562	+	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
OH532_RS27120 (34859)	34859..35680	-	822	WP_001234445.1	DUF932 domain-containing protein	-
OH532_RS27125 (35791)	35791..36087	-	297	WP_001272251.1	hypothetical protein	-
OH532_RS27130 (36387)	36387..36683	+	297	Protein_41	hypothetical protein	-
OH532_RS27135 (37002)	37002..37127	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
OH532_RS27140 (37069)	37069..37218	-	150	Protein_43	plasmid maintenance protein Mok	-
- (37204)	37204..37428	-	225	NuclAT_0	-	Antitoxin
- (37204)	37204..37428	-	225	NuclAT_0	-	Antitoxin
- (37204)	37204..37428	-	225	NuclAT_0	-	Antitoxin
- (37204)	37204..37428	-	225	NuclAT_0	-	Antitoxin
OH532_RS27145 (37440)	37440..38159	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
OH532_RS27150 (38156)	38156..38590	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
OH532_RS27155 (38659)	38659..40682	-	2024	Protein_46	ParB/RepB/Spo0J family partition protein	-
OH532_RS27160 (40743)	40743..40976	-	234	WP_000006003.1	DUF905 family protein	-
OH532_RS27165 (41034)	41034..41561	-	528	WP_000290834.1	single-stranded DNA-binding protein	-
OH532_RS27170 (41863)	41863..42324	+	462	Protein_49	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	rmtB / blaTEM-1B / blaCTX-M-65 / blaKPC-2	-	1..155820	155820

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T261003 WP_001372321.1 NZ_CP107380:c37127-37002 [Klebsiella pneumoniae]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

Antitoxin

Nucleotide

• Length: 225 bp

>AT261003 NZ_CP107380:c37428-37204 [Klebsiella pneumoniae]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin