Detailed information of TA system TA260954

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 35745..36171
• Replicon: plasmid unnamed1
• Accession: NZ_CP107368
• Organism: Klebsiella pneumoniae strain CRKP_32

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: OH524_RS27050
• Protein ID: WP_001372321.1
• Coordinates: 35745..35870 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	35947..36171 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OH524_RS27015 (30800)	30800..31027	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
OH524_RS27020 (31121)	31121..31807	-	687	WP_015059009.1	PAS domain-containing protein	-
OH524_RS27025 (31998)	31998..32381	-	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
OH524_RS27030 (32658)	32658..33305	+	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
OH524_RS27035 (33602)	33602..34423	-	822	WP_001234445.1	DUF932 domain-containing protein	-
OH524_RS27040 (34534)	34534..34830	-	297	WP_001272251.1	hypothetical protein	-
OH524_RS27045 (35130)	35130..35426	+	297	Protein_39	hypothetical protein	-
OH524_RS27050 (35745)	35745..35870	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
OH524_RS27055 (35812)	35812..35961	-	150	Protein_41	plasmid maintenance protein Mok	-
- (35947)	35947..36171	-	225	NuclAT_0	-	Antitoxin
- (35947)	35947..36171	-	225	NuclAT_0	-	Antitoxin
- (35947)	35947..36171	-	225	NuclAT_0	-	Antitoxin
- (35947)	35947..36171	-	225	NuclAT_0	-	Antitoxin
OH524_RS27060 (36183)	36183..36902	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
OH524_RS27065 (36899)	36899..37333	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
OH524_RS27070 (37402)	37402..39425	-	2024	Protein_44	ParB/RepB/Spo0J family partition protein	-
OH524_RS27075 (39486)	39486..39719	-	234	WP_000006003.1	DUF905 family protein	-
OH524_RS27080 (39777)	39777..40304	-	528	WP_000290834.1	single-stranded DNA-binding protein	-
OH524_RS27085 (40606)	40606..41073	+	468	Protein_47	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCTX-M-65 / catA2	-	1..152729	152729

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T260954 WP_001372321.1 NZ_CP107368:c35870-35745 [Klebsiella pneumoniae]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

Antitoxin

Nucleotide

• Length: 225 bp

>AT260954 NZ_CP107368:c36171-35947 [Klebsiella pneumoniae]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin