Detailed information of TA system TA260909

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 37279..37705
• Replicon: plasmid unnamed1
• Accession: NZ_CP107360
• Organism: Klebsiella pneumoniae strain CRKP_34

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: OH518_RS27130
• Protein ID: WP_001372321.1
• Coordinates: 37279..37404 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	37481..37705 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OH518_RS27095 (32755)	32755..33138	-	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
OH518_RS27100 (33415)	33415..34062	+	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
OH518_RS27105 (34359)	34359..34664	-	306	Protein_38	DUF932 domain-containing protein	-
OH518_RS27115 (35448)	35448..35957	-	510	Protein_40	DUF932 domain-containing protein	-
OH518_RS27120 (36068)	36068..36364	-	297	WP_001272251.1	hypothetical protein	-
OH518_RS27125 (36664)	36664..36960	+	297	Protein_42	hypothetical protein	-
OH518_RS27130 (37279)	37279..37404	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
OH518_RS27135 (37346)	37346..37495	-	150	Protein_44	plasmid maintenance protein Mok	-
- (37481)	37481..37705	-	225	NuclAT_0	-	Antitoxin
- (37481)	37481..37705	-	225	NuclAT_0	-	Antitoxin
- (37481)	37481..37705	-	225	NuclAT_0	-	Antitoxin
- (37481)	37481..37705	-	225	NuclAT_0	-	Antitoxin
OH518_RS27140 (37717)	37717..38436	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
OH518_RS27145 (38433)	38433..38867	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
OH518_RS27150 (38936)	38936..40959	-	2024	Protein_47	ParB/RepB/Spo0J family partition protein	-
OH518_RS27155 (41020)	41020..41253	-	234	WP_000006003.1	DUF905 family protein	-
OH518_RS27160 (41311)	41311..41838	-	528	WP_000290834.1	single-stranded DNA-binding protein	-
OH518_RS27165 (42140)	42140..42613	+	474	Protein_50	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	rmtB / blaTEM-1B / blaCTX-M-65 / blaKPC-2	-	1..155508	155508
	-	flank	IS/Tn	-	-	34680..35057	377

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T260909 WP_001372321.1 NZ_CP107360:c37404-37279 [Klebsiella pneumoniae]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

Antitoxin

Nucleotide

• Length: 225 bp

>AT260909 NZ_CP107360:c37705-37481 [Klebsiella pneumoniae]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin