Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 1186665..1187262 Replicon chromosome
Accession NZ_CP107352
Organism Klebsiella pneumoniae strain CRKP_35

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag OH515_RS05660 Protein ID WP_004142563.1
Coordinates 1186945..1187262 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag OH515_RS05655 Protein ID WP_004142561.1
Coordinates 1186665..1186952 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OH515_RS05625 (1182745) 1182745..1182993 + 249 WP_002893037.1 DUF1158 domain-containing protein -
OH515_RS05630 (1183011) 1183011..1183352 - 342 WP_004848570.1 RamA family antibiotic efflux transcriptional regulator -
OH515_RS05635 (1183383) 1183383..1184498 - 1116 WP_012737592.1 MBL fold metallo-hydrolase -
OH515_RS05640 (1184678) 1184678..1185259 + 582 WP_229408964.1 TetR/AcrR family transcriptional regulator -
OH515_RS05645 (1185259) 1185259..1185627 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
OH515_RS05650 (1185747) 1185747..1186400 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
OH515_RS05655 (1186665) 1186665..1186952 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
OH515_RS05660 (1186945) 1186945..1187262 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
OH515_RS05665 (1187447) 1187447..1188490 - 1044 WP_065810347.1 DUF2157 domain-containing protein -
OH515_RS05670 (1189160) 1189160..1190026 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
OH515_RS05675 (1190135) 1190135..1191562 + 1428 WP_004176980.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T260874 WP_004142563.1 NZ_CP107352:c1187262-1186945 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT260874 WP_004142561.1 NZ_CP107352:c1186952-1186665 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References