260504

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 6094606..6095381 Replicon chromosome
Accession NZ_CP107064
Organism Pseudomonas aeruginosa strain PALA20

Toxin (Protein)


Gene name yhaV Uniprot ID V6ADY6
Locus tag PALA20_RS28685 Protein ID WP_009518525.1
Coordinates 6094606..6095064 (-) Length 153 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID V6AEP7
Locus tag PALA20_RS28690 Protein ID WP_023098543.1
Coordinates 6095064..6095381 (-) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PALA20_RS28660 (PALA20_05665) 6089947..6090894 + 948 WP_009518520.1 TIGR03756 family integrating conjugative element protein -
PALA20_RS28665 (PALA20_05666) 6090904..6092298 + 1395 WP_227337149.1 integrating conjugative element protein -
PALA20_RS28670 (PALA20_05667) 6092295..6092654 + 360 WP_009518522.1 hypothetical protein -
PALA20_RS28675 (PALA20_05668) 6092669..6094186 + 1518 WP_009518523.1 conjugal transfer protein TraG N-terminal domain-containing protein -
PALA20_RS28680 (PALA20_05669) 6094202..6094579 - 378 WP_009518524.1 DUF3742 family protein -
PALA20_RS28685 (PALA20_05670) 6094606..6095064 - 459 WP_009518525.1 type II toxin-antitoxin system YhaV family toxin Toxin
PALA20_RS28690 (PALA20_05671) 6095064..6095381 - 318 WP_023098543.1 type II toxin-antitoxin system PrlF family antitoxin Antitoxin
PALA20_RS28695 (PALA20_05672) 6095681..6097498 + 1818 WP_023098542.1 MobH family relaxase -
PALA20_RS28700 (PALA20_05673) 6097480..6097626 - 147 WP_023098541.1 MerR family DNA-binding transcriptional regulator -
PALA20_RS28705 (PALA20_05674) 6097775..6098686 - 912 WP_023098540.1 NAD(P)H-binding protein -
PALA20_RS28710 (PALA20_05675) 6098823..6100208 + 1386 Protein_5674 efflux RND transporter permease subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 6037524..6165828 128304


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(8-144)

Antitoxin

PrlF antitoxin

(8-100)


Sequences

Toxin        


Download         Length: 153 a.a.        Molecular weight: 17660.02 Da        Isoelectric Point: 10.1848

>T260504 WP_009518525.1 NZ_CP107064:c6095064-6094606 [Pseudomonas aeruginosa]
MQRHGWTLLFHDCVIEQLQKLHAAARRAQENDPAGFESNANVKLFRALSQLMLDVVPGDPARDEYRQGNTLGPAHRHWRR
AKIGRRFRLFFRYDSKAKVIVYAWVNDEQTLRSSGSKSDPYVVFEKMLGRGNPPDDWHALIQASKQDWSKLE

Download         Length: 459 bp

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11170.72 Da        Isoelectric Point: 4.7182

>AT260504 WP_023098543.1 NZ_CP107064:c6095381-6095064 [Pseudomonas aeruginosa]
MPAIHEVATLTSKGQITLPKPIRQALGVDAGGKLAFDLRGSEVVVTRVDAEHEDPAIGAFLSLLARDIEAGRNVRGLPED
LARAMLEHAGHGVNLDEEIDGKVEL

Download         Length: 318 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V6ADY6

Antitoxin

Source ID Structure
AlphaFold DB V6AEP7

References