Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3753515..3754209 Replicon chromosome
Accession NZ_CP107000
Organism Escherichia coli strain SY22B2

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag OFB04_RS18480 Protein ID WP_001263489.1
Coordinates 3753515..3753913 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag OFB04_RS18485 Protein ID WP_000554758.1
Coordinates 3753916..3754209 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (3749103) 3749103..3749183 - 81 NuclAT_12 - -
- (3749103) 3749103..3749183 - 81 NuclAT_12 - -
- (3749103) 3749103..3749183 - 81 NuclAT_12 - -
- (3749103) 3749103..3749183 - 81 NuclAT_12 - -
OFB04_RS18455 (3749779) 3749779..3750237 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
OFB04_RS18460 (3750498) 3750498..3751955 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
OFB04_RS18465 (3752012) 3752012..3752533 - 522 Protein_3612 peptide chain release factor H -
OFB04_RS18470 (3752529) 3752529..3752735 - 207 Protein_3613 RtcB family protein -
OFB04_RS18475 (3753053) 3753053..3753505 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
OFB04_RS18480 (3753515) 3753515..3753913 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
OFB04_RS18485 (3753916) 3753916..3754209 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
OFB04_RS18490 (3754261) 3754261..3755316 - 1056 WP_001226164.1 DNA polymerase IV -
OFB04_RS18495 (3755387) 3755387..3756172 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
OFB04_RS18500 (3756144) 3756144..3757856 + 1713 Protein_3619 flagellar biosynthesis protein FlhA -
OFB04_RS18505 (3758080) 3758080..3758577 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T260272 WP_001263489.1 NZ_CP107000:c3753913-3753515 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT260272 WP_000554758.1 NZ_CP107000:c3754209-3753916 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References