Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3759712..3760253 Replicon chromosome
Accession NZ_CP106998
Organism Escherichia coli strain SY221

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag OFB08_RS18520 Protein ID WP_000615983.1
Coordinates 3759975..3760253 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag OFB08_RS18515 Protein ID WP_000729703.1
Coordinates 3759712..3759972 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OFB08_RS18495 (3755387) 3755387..3756172 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
OFB08_RS18500 (3756144) 3756144..3757856 + 1713 Protein_3619 flagellar biosynthesis protein FlhA -
OFB08_RS18505 (3758080) 3758080..3758577 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
OFB08_RS18510 (3758753) 3758753..3759502 - 750 WP_000056849.1 C40 family peptidase -
OFB08_RS18515 (3759712) 3759712..3759972 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
OFB08_RS18520 (3759975) 3759975..3760253 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
OFB08_RS18525 (3760409) 3760409..3761149 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
OFB08_RS18530 (3761120) 3761120..3761887 - 768 WP_012305459.1 class II glutamine amidotransferase -
OFB08_RS18535 (3762093) 3762093..3762671 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T260227 WP_000615983.1 NZ_CP106998:3759975-3760253 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT260227 WP_000729703.1 NZ_CP106998:3759712-3759972 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References