26014

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 11786..12443 Replicon plasmid pKPHS3
Accession NC_016839
Organism Klebsiella pneumoniae subsp. pneumoniae HS11286

Toxin (Protein)


Gene name higB Uniprot ID A0A0H3H633
Locus tag KPHS_p300240 Protein ID YP_005220972.1
Coordinates 12093..12443 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0H3GZA5
Locus tag KPHS_p300230 Protein ID YP_005220971.1
Coordinates 11786..12088 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPHS_p300130 7411..7839 + 429 YP_005220961.1 putative lipoprotein -
KPHS_p300140 7896..8255 + 360 YP_005220962.1 hypothetical protein -
KPHS_p300150 8255..8701 + 447 YP_005220963.1 Fe3+-siderophore ABC transporter permease -
KPHS_p300160 8698..9216 + 519 YP_005220964.1 formate-dependent nitrite reductase -
KPHS_p300170 9216..9446 + 231 YP_005220965.1 hypothetical protein -
KPHS_p300180 9433..10290 + 858 YP_005220966.1 hypothetical protein -
KPHS_p300190 10358..10504 + 147 YP_005220967.1 hypothetical protein -
KPHS_p300200 10521..11048 + 528 YP_005220968.1 SNase-like micrococcal nuclease -
KPHS_p300210 11106..11378 + 273 YP_005220969.1 DNA-binding protein HU-beta -
KPHS_p300220 11466..11759 + 294 YP_005220970.1 putative plasmid-like protein -
KPHS_p300230 11786..12088 - 303 YP_005220971.1 Cro-like protein Antitoxin
KPHS_p300240 12093..12443 - 351 YP_005220972.1 hypothetical protein Toxin
KPHS_p300250 12606..13154 + 549 YP_005220973.1 transcriptional regulator -
KPHS_p300270 13276..13467 - 192 YP_005220974.1 hypothetical protein -
KPHS_p300260 13495..13689 + 195 YP_005220975.1 hypothetical protein -
KPHS_p300280 13700..14071 + 372 YP_005220976.1 hypothetical protein -
KPHS_p300290 14064..14534 + 471 YP_005220977.1 hypothetical protein -
KPHS_p300300 14549..14884 - 336 YP_005220978.1 hypothetical protein -
KPHS_p300310 14981..15469 + 489 YP_005220979.1 hypothetical protein -
KPHS_p300320 15472..15969 + 498 YP_005220980.1 hypothetical protein -
KPHS_p300330 16166..16336 + 171 YP_005220981.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaTEM-1B / rmtB / tet(G) / floR / qacE / aadA2 / dfrA12 / aac(3)-IId / aph(6)-Id / aph(3'')-Ib / sul2 / blaCTX-M-14 - 1..105974 105974


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-106)

Antitoxin

Cro/C1-type helix-turn-helix domain

(33-79)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13328.21 Da        Isoelectric Point: 5.2421

>T26014 YP_005220972.1 NC_016839:c12443-12093 [Klebsiella pneumoniae subsp. pneumoniae HS11286]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Download         Length: 351 bp

>T26014 NC_016839:c12443-12093 [Klebsiella pneumoniae subsp. pneumoniae HS11286]
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11006.73 Da        Isoelectric Point: 7.2036

>AT26014 YP_005220971.1 NC_016839:c12088-11786 [Klebsiella pneumoniae subsp. pneumoniae HS11286]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Download         Length: 303 bp

>AT26014 NC_016839:c12088-11786 [Klebsiella pneumoniae subsp. pneumoniae HS11286]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H633

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZA5

References