Detailed information of TA system TA260063

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 56479..56896
• Replicon: plasmid pMFDS1016424
• Accession: NZ_CP106925
• Organism: Escherichia coli strain MFDS1016424

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: OEG87_RS23805
• Protein ID: WP_001372321.1
• Coordinates: 56479..56604 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	56702..56896 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OEG87_RS23785 (53027)	53027..53399	-	373	Protein_53	hypothetical protein	-
OEG87_RS23790 (53546)	53546..54217	+	672	Protein_54	IS66-like element accessory protein TnpA	-
OEG87_RS23795 (54222)	54222..54569	+	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
OEG87_RS23800 (54589)	54589..56160	+	1572	WP_099554548.1	IS66 family transposase	-
OEG87_RS23805 (56479)	56479..56604	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
OEG87_RS23810 (56546)	56546..56695	-	150	Protein_58	plasmid maintenance protein Mok	-
- (56702)	56702..56896	-	195	NuclAT_0	-	Antitoxin
- (56702)	56702..56896	-	195	NuclAT_0	-	Antitoxin
- (56702)	56702..56896	-	195	NuclAT_0	-	Antitoxin
- (56702)	56702..56896	-	195	NuclAT_0	-	Antitoxin
OEG87_RS23815 (56820)	56820..57627	-	808	Protein_59	plasmid SOS inhibition protein A	-
OEG87_RS23820 (57624)	57624..58058	-	435	WP_000845954.1	conjugation system SOS inhibitor PsiB	-
OEG87_RS23825 (58113)	58113..60076	-	1964	Protein_61	ParB/RepB/Spo0J family partition protein	-
OEG87_RS23830 (60138)	60138..60698	+	561	Protein_62	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	eltA / eltB / cssA	1..88935	88935
	-	inside	IScluster/Tn	-	cssA	53576..66837	13261

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T260063 WP_001372321.1 NZ_CP106925:c56604-56479 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

Antitoxin

Nucleotide

• Length: 195 bp

>AT260063 NZ_CP106925:c56896-56702 [Escherichia coli]
TCACGCGGATTTCCCGTAGCCTGAATGAGCGTATTCTTTTCAGGAAAAGTGAATGTGGCCAGCGTGCAGGGATATGAGCT
ATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGCAGAA
AGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin