Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 6220451..6221123 Replicon chromosome
Accession NC_016830
Organism Pseudomonas fluorescens F113

Toxin (Protein)


Gene name higB Uniprot ID A0A0F4XLD8
Locus tag PSF113_RS57715 Protein ID WP_024616744.1
Coordinates 6220758..6221123 (-) Length 122 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0F4XJJ2
Locus tag PSF113_RS57710 Protein ID WP_024616743.1
Coordinates 6220451..6220765 (-) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PSF113_RS57690 6215823..6216542 + 720 WP_014340528.1 protocatechuate 3,4-dioxygenase subunit beta -
PSF113_RS57695 6216555..6217157 + 603 WP_014340529.1 protocatechuate 3,4-dioxygenase subunit alpha -
PSF113_RS57700 6217512..6219830 + 2319 WP_041476430.1 hypothetical protein -
PSF113_RS57705 6219867..6220328 + 462 WP_014340531.1 Lrp/AsnC family transcriptional regulator -
PSF113_RS57710 6220451..6220765 - 315 WP_024616743.1 XRE family transcriptional regulator Antitoxin
PSF113_RS57715 6220758..6221123 - 366 WP_024616744.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
PSF113_RS57720 6221485..6223167 + 1683 WP_014340534.1 NAD(P)/FAD-dependent oxidoreductase -
PSF113_RS57725 6223180..6223974 + 795 WP_014340535.1 carbon-nitrogen hydrolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(14-106)

Antitoxin

Cro/C1-type helix-turn-helix domain

(33-86)


Sequences

Toxin        


Download         Length: 122 a.a.        Molecular weight: 14256.94 Da        Isoelectric Point: 5.2125

>T26003 WP_024616744.1 NC_016830:c6221123-6220758 [Pseudomonas fluorescens F113]
MKWDVEYTDEFELWWDRLSESEQDSVQVSVMLLGDTGPYLGFPHTSDIKGSRHGNLRELRVQHAGRPYRVLYAFDPRRCA
ILLIGGDKTGQDRWYQQYVPLAERLYDEHLEVLKREGFDHG

Download         Length: 366 bp

>T26003 NC_016830:c6221123-6220758 [Pseudomonas fluorescens F113]
ATGAAATGGGACGTTGAATACACTGACGAATTTGAGTTGTGGTGGGACCGTCTCAGTGAGTCGGAGCAAGACTCGGTACA
AGTCTCGGTGATGCTACTCGGCGATACCGGGCCGTATCTGGGCTTTCCTCACACCAGTGATATCAAAGGTTCGCGTCATG
GCAACCTTCGTGAGTTACGGGTGCAACATGCAGGTAGGCCTTATCGTGTGCTCTATGCGTTTGATCCACGACGCTGCGCC
ATCCTGCTGATCGGGGGAGACAAAACCGGCCAGGACCGTTGGTATCAGCAATATGTGCCTTTGGCCGAACGCCTCTATGA
CGAGCATTTGGAAGTATTGAAGAGAGAGGGCTTTGACCATGGCTAA

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 11899.64 Da        Isoelectric Point: 9.6213

>AT26003 WP_024616743.1 NC_016830:c6220765-6220451 [Pseudomonas fluorescens F113]
MAKKFSDLMARRSPDSQARAQALYQQLRAEMPLHELRQAQELSQEALAKALHINQAAVSKMERRTDMYISTLRNYIRAMG
GELEIIATFPEGQVRIENFSHPTP

Download         Length: 315 bp

>AT26003 NC_016830:c6220765-6220451 [Pseudomonas fluorescens F113]
ATGGCTAAGAAATTTTCCGATTTGATGGCGCGTCGTTCGCCTGACTCCCAGGCTCGTGCACAGGCTCTGTATCAGCAGTT
GCGTGCGGAAATGCCGCTGCACGAGTTGCGTCAGGCGCAGGAGTTGAGCCAGGAGGCCTTGGCCAAGGCACTGCATATCA
ACCAGGCGGCAGTCTCGAAAATGGAGCGCCGTACGGACATGTACATCAGCACATTGCGCAACTACATCCGGGCCATGGGG
GGGGAATTGGAGATTATCGCGACCTTTCCGGAGGGGCAGGTCAGGATCGAAAATTTCTCCCATCCAACACCGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0F4XLD8

Antitoxin

Source ID Structure
AlphaFold DB A0A0F4XJJ2

References