Detailed information of TA system TA026002

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 5618159..5618814
• Replicon: chromosome
• Accession: NC_016830
• Organism: Pseudomonas fluorescens F113

Toxin (Protein)

• Gene name: higB
• Uniprot ID: G8QAX9
• Locus tag: PSF113_RS54945
• Protein ID: WP_013694185.1
• Coordinates: 5618470..5618814 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: PSF113_RS54940
• Protein ID: WP_003205391.1
• Coordinates: 5618159..5618473 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PSF113_RS54920	5613802..5614977	-	1176	WP_041476134.1	aspartate aminotransferase family protein	-
PSF113_RS54925	5615077..5615994	+	918	WP_014340099.1	LysR family transcriptional regulator	-
PSF113_RS54930	5616124..5617137	+	1014	WP_014340100.1	diguanylate cyclase	-
PSF113_RS54935	5617242..5617946	+	705	WP_014340101.1	hypothetical protein	-
PSF113_RS54940	5618159..5618473	-	315	WP_003205391.1	helix-turn-helix domain-containing protein	Antitoxin
PSF113_RS54945	5618470..5618814	-	345	WP_013694185.1	hypothetical protein	Toxin
PSF113_RS54950	5618954..5619841	-	888	WP_003178131.1	heme o synthase	-
PSF113_RS54955	5619853..5620188	-	336	WP_014340102.1	cytochrome o ubiquinol oxidase subunit IV	-
PSF113_RS54960	5620189..5620815	-	627	WP_014340103.1	cytochrome o ubiquinol oxidase subunit III	-
PSF113_RS54965	5620819..5622849	-	2031	WP_014340104.1	cytochrome o ubiquinol oxidase subunit I	-
PSF113_RS54970	5622853..5623800	-	948	WP_041476135.1	ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-87)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13753.72 Da
• Isoelectric Point: 8.3860

>T26002 WP_013694185.1 NC_016830:c5618814-5618470 [Pseudomonas fluorescens F113]
MRTLFFETTTFTDTVGHYLTDDEYRLLQSYMLQHPEVGDVMPRTGGFRKLRWFDERRGKGKRGGLRVIYYWLMNDRQFWM
FAIYDKDELENLTSEQEKTLKRAIEAELKVRGTL

Nucleotide

• Length: 345 bp

>T26002 NC_016830:c5618814-5618470 [Pseudomonas fluorescens F113]
ATGCGTACCCTATTTTTTGAAACGACTACATTCACCGACACGGTTGGCCATTACCTGACTGATGATGAGTATCGCCTGCT
TCAGTCCTATATGCTGCAGCATCCAGAGGTTGGTGACGTCATGCCACGTACCGGCGGCTTTCGTAAGCTGCGCTGGTTTG
ATGAACGTCGTGGTAAAGGGAAGCGAGGCGGGTTGCGCGTTATTTACTATTGGCTCATGAACGATCGTCAGTTCTGGATG
TTTGCGATATATGACAAGGATGAGCTGGAAAACCTGACTTCCGAGCAAGAGAAAACGCTCAAGCGCGCCATAGAAGCTGA
GTTGAAAGTACGAGGTACCTTATGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11771.76 Da
• Isoelectric Point: 9.8409

>AT26002 WP_003205391.1 NC_016830:c5618473-5618159 [Pseudomonas fluorescens F113]
MKKRDLFAEMMQGVEEMAAHREGKITLRQISIEDKPVPEVSAQEIVALRDKLHMSQAVFAKSIRTSPGTLRNWEQEKSKP
NAQAALLIKLVEKFPDMVERLGAV

Nucleotide

• Length: 315 bp

>AT26002 NC_016830:c5618473-5618159 [Pseudomonas fluorescens F113]
ATGAAAAAGCGCGATCTGTTTGCCGAGATGATGCAAGGCGTCGAGGAGATGGCCGCTCACCGGGAGGGCAAGATTACCCT
TCGCCAGATATCGATTGAAGACAAACCGGTTCCCGAGGTGTCTGCCCAGGAAATCGTGGCGTTGCGCGACAAGCTCCACA
TGTCTCAAGCCGTTTTCGCCAAGAGCATCAGGACCAGTCCAGGTACACTCAGGAACTGGGAGCAGGAAAAATCCAAGCCT
AACGCCCAGGCGGCTTTGTTGATCAAACTGGTCGAGAAGTTTCCAGATATGGTCGAACGGCTTGGTGCTGTTTGA

Structures

Toxin

• AlphaFold DB: G8QAX9