Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 31734..32464 Replicon chromosome
Accession NZ_CP106894
Organism Enterobacter hormaechei strain EHX_PAT_001

Toxin (Protein)


Gene name higB Uniprot ID A0A837FIL2
Locus tag N6139_RS00155 Protein ID WP_023302825.1
Coordinates 31734..32048 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A837FJX0
Locus tag N6139_RS00160 Protein ID WP_015570115.1
Coordinates 32048..32464 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N6139_RS00130 (N6139_00130) 27400..28485 + 1086 Protein_25 cellulase family glycosylhydrolase -
N6139_RS00135 (N6139_00135) 28391..29575 - 1185 WP_048245027.1 multidrug efflux MFS transporter EmrD -
N6139_RS00140 (N6139_00140) 29751..30584 - 834 WP_023323922.1 DMT family transporter -
N6139_RS00145 (N6139_00145) 30647..31093 - 447 WP_003861064.1 GNAT family N-acetyltransferase -
N6139_RS00150 (N6139_00150) 31158..31247 - 90 WP_022646551.1 type I toxin-antitoxin system toxin TisB -
N6139_RS00155 (N6139_00155) 31734..32048 + 315 WP_023302825.1 type II toxin-antitoxin system HigB family toxin Toxin
N6139_RS00160 (N6139_00160) 32048..32464 + 417 WP_015570115.1 helix-turn-helix domain-containing protein Antitoxin
N6139_RS00165 (N6139_00165) 32611..32709 + 99 WP_057979964.1 ilvB operon leader peptide IvbL -
N6139_RS00170 (N6139_00170) 32939..34627 + 1689 WP_003861056.1 acetolactate synthase large subunit -
N6139_RS00175 (N6139_00175) 34631..34918 + 288 WP_003861055.1 acetolactate synthase small subunit -
N6139_RS00180 (N6139_00180) 35001..35594 + 594 WP_003861054.1 transcriptional regulator UhpA -
N6139_RS00185 (N6139_00185) 35591..37096 + 1506 WP_003861052.1 signal transduction histidine-protein kinase/phosphatase UhpB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(21-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(83-136)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12415.51 Da        Isoelectric Point: 10.2825

>T259969 WP_023302825.1 NZ_CP106894:31734-32048 [Enterobacter hormaechei]
MHLISMKAILDAVSQFPQHREELLFLGRVIEKSHCPTPAALRKLFPTLDNFKYLDKHYVIDIANNNLRVVALIFFESQKF
YVRHVFTHKEYDRFTEKHRTKGKK

Download         Length: 315 bp

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15367.95 Da        Isoelectric Point: 5.7670

>AT259969 WP_015570115.1 NZ_CP106894:32048-32464 [Enterobacter hormaechei]
MIVADAMKATHALVAAVPLLGEQPSEKDYKDALELVEYLLMNEPNSPLLDIVCARISRYEANRPEIVALRKEMESVPVGI
AVLRTLMDQYNLTISDFKDEIGSKSMVSRVLNGQRQLTLNHIKKLAARFGVSPALFIE

Download         Length: 417 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A837FIL2

Antitoxin

Source ID Structure
AlphaFold DB A0A837FJX0

References