Detailed information of TA system TA025994

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3136955..3137682
• Replicon: chromosome
• Accession: NC_016822
• Organism: Shigella sonnei 53G

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: SSON53G_RS16695
• Protein ID: WP_000547563.1
• Coordinates: 3137371..3137682 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: SSON53G_RS16690
• Protein ID: WP_000126296.1
• Coordinates: 3136955..3137374 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SSON53G_RS16670 (3132006)	3132006..3132533	-	528	WP_001078777.1	electron transport protein HydN	-
SSON53G_RS16675 (3132681)	3132681..3133691	-	1011	WP_001402444.1	DNA-binding transcriptional regulator AscG	-
SSON53G_RS16680 (3133951)	3133951..3135408	+	1458	WP_001107890.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
SSON53G_RS16685 (3135417)	3135417..3136841	+	1425	WP_000110319.1	6-phospho-beta-glucosidase AscB	-
SSON53G_RS16690 (3136955)	3136955..3137374	-	420	WP_000126296.1	helix-turn-helix domain-containing protein	Antitoxin
SSON53G_RS16695 (3137371)	3137371..3137682	-	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
SSON53G_RS16700 (3137844)	3137844..3138617	-	774	WP_001026444.1	hypothetical protein	-
SSON53G_RS16705 (3138642)	3138642..3139112	-	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
SSON53G_RS16710 (3139105)	3139105..3139515	-	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
SSON53G_RS16715 (3139512)	3139512..3140279	-	768	WP_000067403.1	formate hydrogenlyase subunit HycG	-
SSON53G_RS16720 (3140279)	3140279..3140821	-	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
SSON53G_RS16725 (3140831)	3140831..3142528	-	1698	WP_001288143.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T25994 WP_000547563.1 NC_016822:c3137682-3137371 [Shigella sonnei 53G]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T25994 NC_016822:c3137682-3137371 [Shigella sonnei 53G]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAGATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTAGGTGGGAACAACCTAAGGGTGATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACTAATCACGCCGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.38 Da
• Isoelectric Point: 4.3653

>AT25994 WP_000126296.1 NC_016822:c3137374-3136955 [Shigella sonnei 53G]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT25994 NC_016822:c3137374-3136955 [Shigella sonnei 53G]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGTAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACCAAGAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGCGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22