Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 294960..295501 Replicon chromosome
Accession NC_016822
Organism Shigella sonnei 53G

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag SSON53G_RS01420 Protein ID WP_000615976.1
Coordinates 294960..295238 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag SSON53G_RS01425 Protein ID WP_000729704.1
Coordinates 295241..295501 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SSON53G_RS01405 (292542) 292542..293120 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
SSON53G_RS01410 (293326) 293326..294093 + 768 WP_000333380.1 class II glutamine amidotransferase -
SSON53G_RS01415 (294064) 294064..294804 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
SSON53G_RS01420 (294960) 294960..295238 - 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
SSON53G_RS01425 (295241) 295241..295501 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
SSON53G_RS01430 (295687) 295687..296460 + 774 WP_127148796.1 C40 family peptidase -
SSON53G_RS33290 (297501) 297501..297629 + 129 Protein_269 ISAs1 family transposase -
SSON53G_RS01440 (297700) 297700..298197 + 498 WP_000006258.1 REP-associated tyrosine transposase RayT -
SSON53G_RS01445 (298320) 298320..300032 - 1713 Protein_271 flagellar biosynthesis protein FlhA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 283543..298197 14654


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T25981 WP_000615976.1 NC_016822:c295238-294960 [Shigella sonnei 53G]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T25981 NC_016822:c295238-294960 [Shigella sonnei 53G]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT25981 WP_000729704.1 NC_016822:c295501-295241 [Shigella sonnei 53G]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT25981 NC_016822:c295501-295241 [Shigella sonnei 53G]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCTGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References