Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4199558..4200285 Replicon chromosome
Accession NZ_CP106756
Organism Escherichia coli strain B-JRPT-4119

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag N8153_RS20380 Protein ID WP_000550189.1
Coordinates 4199971..4200285 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag N8153_RS20375 Protein ID WP_000560266.1
Coordinates 4199558..4199974 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N8153_RS20365 (4194718) 4194718..4197069 + 2352 WP_000695486.1 alpha-glucosidase -
N8153_RS20370 (4197495) 4197495..4199513 + 2019 WP_000121449.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
N8153_RS20375 (4199558) 4199558..4199974 - 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
N8153_RS20380 (4199971) 4199971..4200285 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
N8153_RS20385 (4200569) 4200569..4201705 - 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
N8153_RS20390 (4201790) 4201790..4202293 + 504 WP_001300832.1 M48 family metallopeptidase -
N8153_RS20395 (4202370) 4202370..4203062 + 693 WP_000942546.1 vancomycin high temperature exclusion protein -
N8153_RS20400 (4203141) 4203141..4204127 + 987 WP_001301393.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T259726 WP_000550189.1 NZ_CP106756:c4200285-4199971 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT259726 WP_000560266.1 NZ_CP106756:c4199974-4199558 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References