Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 1198313..1199007 Replicon chromosome
Accession NZ_CP106756
Organism Escherichia coli strain B-JRPT-4119

Toxin (Protein)


Gene name yafO Uniprot ID S1EXB8
Locus tag N8153_RS05700 Protein ID WP_001263493.1
Coordinates 1198609..1199007 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1FJN6
Locus tag N8153_RS05695 Protein ID WP_000554757.1
Coordinates 1198313..1198606 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N8153_RS05675 (1193953) 1193953..1194450 + 498 WP_000006260.1 REP-associated tyrosine transposase RayT -
N8153_RS05680 (1194666) 1194666..1196378 - 1713 Protein_1092 flagellar biosynthesis protein FlhA -
N8153_RS05685 (1196350) 1196350..1197135 + 786 WP_000207564.1 putative lateral flagellar export/assembly protein LafU -
N8153_RS05690 (1197206) 1197206..1198261 + 1056 WP_001226164.1 DNA polymerase IV -
N8153_RS05695 (1198313) 1198313..1198606 + 294 WP_000554757.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
N8153_RS05700 (1198609) 1198609..1199007 + 399 WP_001263493.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
N8153_RS05705 (1199017) 1199017..1199469 + 453 WP_001059847.1 GNAT family N-acetyltransferase -
N8153_RS05710 (1199715) 1199715..1199918 + 204 Protein_1098 RtcB family protein -
N8153_RS05715 (1199917) 1199917..1200438 + 522 Protein_1099 peptide chain release factor H -
N8153_RS05720 (1200495) 1200495..1201952 - 1458 WP_001293003.1 cytosol nonspecific dipeptidase -
N8153_RS05725 (1202213) 1202213..1202671 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- (1203267) 1203267..1203347 + 81 NuclAT_12 - -
- (1203267) 1203267..1203347 + 81 NuclAT_12 - -
- (1203267) 1203267..1203347 + 81 NuclAT_12 - -
- (1203267) 1203267..1203347 + 81 NuclAT_12 - -
N8153_RS05730 (1202763) 1202763..1204007 + 1245 WP_000189541.1 esterase FrsA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15472.83 Da        Isoelectric Point: 8.0949

>T259712 WP_001263493.1 NZ_CP106756:1198609-1199007 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.81 Da        Isoelectric Point: 5.9050

>AT259712 WP_000554757.1 NZ_CP106756:1198313-1198606 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1EXB8

Antitoxin

Source ID Structure
AlphaFold DB S1FJN6

References