Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 1192277..1192818 Replicon chromosome
Accession NZ_CP106756
Organism Escherichia coli strain B-JRPT-4119

Toxin (Protein)


Gene name yafQ Uniprot ID A0A140NAP5
Locus tag N8153_RS05660 Protein ID WP_000615981.1
Coordinates 1192277..1192555 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag N8153_RS05665 Protein ID WP_000729704.1
Coordinates 1192558..1192818 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N8153_RS05645 (1189859) 1189859..1190437 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
N8153_RS05650 (1190643) 1190643..1191410 + 768 WP_000333379.1 class II glutamine amidotransferase -
N8153_RS05655 (1191381) 1191381..1192121 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
N8153_RS05660 (1192277) 1192277..1192555 - 279 WP_000615981.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
N8153_RS05665 (1192558) 1192558..1192818 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
N8153_RS05670 (1193004) 1193004..1193777 + 774 WP_001353765.1 C40 family peptidase -
N8153_RS05675 (1193953) 1193953..1194450 + 498 WP_000006260.1 REP-associated tyrosine transposase RayT -
N8153_RS05680 (1194666) 1194666..1196378 - 1713 Protein_1092 flagellar biosynthesis protein FlhA -
N8153_RS05685 (1196350) 1196350..1197135 + 786 WP_000207564.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10860.65 Da        Isoelectric Point: 10.0702

>T259711 WP_000615981.1 NZ_CP106756:c1192555-1192277 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQSSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT259711 WP_000729704.1 NZ_CP106756:c1192818-1192558 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MMG
PDB 4MMJ
PDB 4ML2
PDB 4ML0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References