Detailed information of TA system TA259680

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5850190..5850785
• Replicon: chromosome
• Accession: NZ_CP106744
• Organism: Pseudomonas aeruginosa strain PALA16

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A241XLJ5
• Locus tag: PALA16_RS27530
• Protein ID: WP_003117425.1
• Coordinates: 5850507..5850785 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: PALA16_RS27525
• Protein ID: WP_003099268.1
• Coordinates: 5850190..5850495 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PALA16_RS27495 (PALA16_05455)	5845643..5845933	-	291	WP_023083237.1	DUF5447 family protein	-
PALA16_RS27500 (PALA16_05456)	5846145..5846417	-	273	WP_003115921.1	hypothetical protein	-
PALA16_RS27505 (PALA16_05457)	5846527..5846793	+	267	WP_016852153.1	hypothetical protein	-
PALA16_RS27510 (PALA16_05458)	5846925..5847761	+	837	WP_223656480.1	helix-turn-helix domain-containing protein	-
PALA16_RS27515 (PALA16_05459)	5847736..5849274	+	1539	WP_023082696.1	GNAT family N-acetyltransferase	-
PALA16_RS27520	5849286..5849813	-	528	WP_071535723.1	ATP-binding protein	-
PALA16_RS27525 (PALA16_05460)	5850190..5850495	-	306	WP_003099268.1	HigA family addiction module antitoxin	Antitoxin
PALA16_RS27530	5850507..5850785	-	279	WP_003117425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PALA16_RS27535	5850838..5850966	-	129	Protein_5441	integrase	-
PALA16_RS27540 (PALA16_05461)	5851114..5853342	+	2229	WP_003099265.1	TonB-dependent receptor	-
PALA16_RS27545 (PALA16_05462)	5853412..5854059	-	648	WP_003095021.1	carbonate dehydratase	-
PALA16_RS27550 (PALA16_05463)	5854121..5855359	-	1239	WP_014603324.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10701.28 Da
• Isoelectric Point: 8.5576

>T259680 WP_003117425.1 NZ_CP106744:c5850785-5850507 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T259680 NZ_CP106744:c5850785-5850507 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTAGGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT259680 WP_003099268.1 NZ_CP106744:c5850495-5850190 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT259680 NZ_CP106744:c5850495-5850190 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCCGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: A0A241XLJ5