Detailed information of TA system TA025940

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1458285..1458924
• Replicon: chromosome
• Accession: NC_016809
• Organism: Haemophilus influenzae 10810

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A8B2WM63
• Locus tag: HIB_RS07295
• Protein ID: WP_005694311.1
• Coordinates: 1458285..1458590 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HIB_RS07300
• Protein ID: WP_005650217.1
• Coordinates: 1458601..1458924 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HIB_RS07275	1453714..1455753	-	2040	WP_005646675.1	excinuclease ABC subunit B	-
HIB_RS07285	1456368..1457336	-	969	WP_015702144.1	zinc transporter permease subunit ZevB	-
HIB_RS07290	1457339..1457959	-	621	WP_005650213.1	zinc transporter binding subunit ZevA	-
HIB_RS07295	1458285..1458590	+	306	WP_005694311.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HIB_RS07300	1458601..1458924	+	324	WP_005650217.1	HigA family addiction module antidote protein	Antitoxin
HIB_RS07305	1459087..1460757	+	1671	WP_015702145.1	energy-dependent translational throttle protein EttA	-
HIB_RS07310	1460819..1461160	+	342	WP_005666735.1	SirB2 family protein	-
HIB_RS07315	1461165..1463009	+	1845	WP_015702146.1	tRNA(Met) cytidine acetyltransferase TmcA	-
HIB_RS10340	1463006..1463179	+	174	WP_005652999.1	DUF5363 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-99)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (4-82)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 12248.89 Da
• Isoelectric Point: 8.0244

>T25940 WP_005694311.1 NC_016809:1458285-1458590 [Haemophilus influenzae 10810]
MFNLKREHFRDDYLYRFYQYGDTHSKIPSNLYKVLARKLDMISASENINDLRSPPANHLELLEPKENKIYSIRVNKQYCL
IFKYENNEVNNLYLDPHSYNL

Nucleotide

• Length: 306 bp

>T25940 NC_016809:1458285-1458590 [Haemophilus influenzae 10810]
ATGTTTAATTTAAAGCGTGAGCATTTTAGAGATGATTATCTTTATAGGTTTTATCAATATGGTGATACTCATAGTAAGAT
TCCTTCTAATTTGTATAAAGTATTGGCAAGAAAATTAGATATGATAAGTGCCTCAGAAAATATCAACGATTTACGTTCTC
CACCAGCTAACCATTTGGAGTTGCTTGAGCCAAAAGAAAATAAAATTTATTCCATTCGAGTGAATAAACAATATTGTTTA
ATTTTTAAATATGAAAATAACGAAGTAAATAATTTATATTTAGATCCACATTCTTATAATTTGTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 12347.17 Da
• Isoelectric Point: 6.8804

>AT25940 WP_005650217.1 NC_016809:1458601-1458924 [Haemophilus influenzae 10810]
MMTRKPTSVGEILQEEFLEPLSLKISDLAQILDVHRNTASNIVNNSSRITLEMAVKLAKVFDTTPEFWLNLQTRIDLWDL
EHNKRFQQSLANVKPALHRHDTSTFAM

Nucleotide

• Length: 324 bp

>AT25940 NC_016809:1458601-1458924 [Haemophilus influenzae 10810]
ATGATGACGAGAAAACCCACCTCTGTTGGTGAAATTCTACAAGAAGAGTTTTTAGAACCTTTAAGTCTTAAAATTAGTGA
TTTAGCTCAAATTTTAGATGTACATCGTAATACGGCAAGCAATATTGTTAATAATAGTTCACGAATTACGCTAGAAATGG
CAGTTAAACTTGCTAAAGTATTTGATACTACGCCTGAGTTTTGGCTTAATCTGCAAACACGTATTGATTTATGGGACCTT
GAACACAATAAACGTTTTCAACAAAGTCTTGCAAATGTTAAGCCCGCTCTACATCGGCATGATACTTCTACATTTGCAAT
GTAA

Structures

Toxin

• AlphaFold DB: A0A8B2WM63