Detailed information of TA system TA025933

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 372400..373069
• Replicon: chromosome
• Accession: NC_016809
• Organism: Haemophilus influenzae 10810

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A2R3G0F4
• Locus tag: HIB_RS01845
• Protein ID: WP_015701504.1
• Coordinates: 372686..373069 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A502K5Z3
• Locus tag: HIB_RS01840
• Protein ID: WP_005651224.1
• Coordinates: 372400..372702 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HIB_RS10320	367401..367559	-	159	WP_005651234.1	hypothetical protein	-
HIB_RS01815	367762..368097	-	336	WP_015701500.1	phage tail protein	-
HIB_RS01820	368106..371447	-	3342	WP_015701501.1	tape measure protein	-
HIB_RS01825	371526..371774	-	249	WP_015701502.1	hypothetical protein	-
HIB_RS10210	371838..372032	-	195	Protein_339	hypothetical protein	-
HIB_RS01835	372148..372384	-	237	WP_101494098.1	hypothetical protein	-
HIB_RS01840	372400..372702	-	303	WP_005651224.1	XRE family transcriptional regulator	Antitoxin
HIB_RS01845	372686..373069	-	384	WP_015701504.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HIB_RS01850	373138..373362	-	225	WP_005651221.1	DUF4035 domain-containing protein	-
HIB_RS01855	373407..373814	-	408	WP_015701505.1	hypothetical protein	-
HIB_RS10215	373830..374084	-	255	WP_015701506.1	hypothetical protein	-
HIB_RS01860	374093..374743	-	651	WP_015701507.1	hypothetical protein	-
HIB_RS01865	374746..375156	-	411	WP_005651206.1	phage tail protein	-
HIB_RS01870	375165..375695	-	531	WP_015701508.1	phage tail protein	-
HIB_RS01875	375698..376009	-	312	WP_015701509.1	hypothetical protein	-
HIB_RS01880	375990..376313	-	324	WP_015701510.1	DUF2190 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	358164..403049	44885

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-110)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-81)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14868.05 Da
• Isoelectric Point: 4.4987

>T25933 WP_015701504.1 NC_016809:c373069-372686 [Haemophilus influenzae 10810]
MKQEWEVILQDPLLNWLETLAEDDVLKIYAALELLSTEGPQLSRPYADTLQGSKYTNLKELRVQSKLSVFRLFYIFDPVR
QAIVLCGGDKKGKKEKLFYKEMIALAEQTYDDYLSELTKEQENEREI

Nucleotide

• Length: 384 bp

>T25933 NC_016809:c373069-372686 [Haemophilus influenzae 10810]
ATGAAACAAGAATGGGAAGTAATTTTACAAGACCCACTTTTGAACTGGTTAGAAACGCTGGCAGAAGATGACGTATTAAA
AATCTATGCGGCGTTGGAATTATTATCAACAGAAGGTCCACAATTAAGCAGACCTTATGCGGATACGCTGCAAGGCTCTA
AATATACCAATTTAAAAGAATTGCGAGTACAGTCTAAATTATCGGTATTCCGTTTATTTTATATTTTCGACCCTGTCAGA
CAAGCGATTGTTTTATGTGGCGGAGATAAAAAAGGCAAGAAAGAAAAACTCTTTTACAAAGAGATGATTGCCCTAGCGGA
ACAAACCTATGATGATTACCTTTCTGAATTAACTAAGGAGCAAGAAAATGAGCGTGAAATTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11258.21 Da
• Isoelectric Point: 9.5655

>AT25933 WP_005651224.1 NC_016809:c372702-372400 [Haemophilus influenzae 10810]
MSVKFKDLMNNLPAEKQAKVKAMADDMRMELQLYRIREELELSQKQMAEALSISQPSVVALEKRGNDIKLSSVKRYIEAM
GGVLNLSVELPTGKTVTFNL

Nucleotide

• Length: 303 bp

>AT25933 NC_016809:c372702-372400 [Haemophilus influenzae 10810]
ATGAGCGTGAAATTTAAAGATCTGATGAATAATCTTCCAGCTGAAAAGCAAGCGAAAGTGAAAGCTATGGCAGACGATAT
GCGAATGGAGTTACAACTTTACCGTATTCGTGAAGAATTAGAGCTTTCACAAAAGCAAATGGCAGAAGCATTAAGTATTT
CTCAGCCGTCAGTTGTCGCCCTTGAAAAACGTGGCAATGACATTAAATTATCATCAGTTAAACGTTACATTGAGGCAATG
GGTGGTGTGTTAAATTTATCGGTTGAATTACCCACAGGAAAAACAGTTACTTTCAACTTATAG

Structures

Toxin

• AlphaFold DB: A0A2R3G0F4

Antitoxin

• AlphaFold DB: A0A502K5Z3