Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 656170..656969 Replicon chromosome
Accession NZ_CP104851
Organism Escherichia coli strain GYX208DH4E-2

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag N6H16_RS03215 Protein ID WP_000347251.1
Coordinates 656170..656634 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag N6H16_RS03220 Protein ID WP_001307405.1
Coordinates 656634..656969 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N6H16_RS03185 (651171) 651171..651605 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
N6H16_RS03190 (651623) 651623..652501 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
N6H16_RS03195 (652491) 652491..653270 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
N6H16_RS03200 (653281) 653281..653754 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
N6H16_RS03205 (653777) 653777..655057 - 1281 WP_000681903.1 tagatose-bisphosphate aldolase subunit KbaZ -
N6H16_RS03210 (655306) 655306..656115 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
N6H16_RS03215 (656170) 656170..656634 - 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
N6H16_RS03220 (656634) 656634..656969 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
N6H16_RS03225 (657118) 657118..658689 - 1572 WP_001273761.1 galactarate dehydratase -
N6H16_RS03230 (659064) 659064..660398 + 1335 WP_000599652.1 galactarate/glucarate/glycerate transporter GarP -
N6H16_RS03235 (660414) 660414..661184 + 771 WP_001058214.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T259321 WP_000347251.1 NZ_CP104851:c656634-656170 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT259321 WP_001307405.1 NZ_CP104851:c656969-656634 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References