Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 1604369..1605048 Replicon chromosome
Accession NZ_CP104848
Organism Escherichia coli strain DFK.1

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag N7218_RS07900 Protein ID WP_000057523.1
Coordinates 1604369..1604671 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag N7218_RS07905 Protein ID WP_000806442.1
Coordinates 1604707..1605048 (+) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N7218_RS07875 (1599745) 1599745..1601397 + 1653 WP_000771748.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
N7218_RS07880 (1601435) 1601435..1601938 - 504 WP_000667000.1 hypothetical protein -
N7218_RS07885 (1601935) 1601935..1602735 - 801 WP_000439798.1 hypothetical protein -
N7218_RS07890 (1602759) 1602759..1603238 - 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
N7218_RS07895 (1603442) 1603442..1604236 - 795 WP_000365147.1 TraB/GumN family protein -
N7218_RS07900 (1604369) 1604369..1604671 + 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
N7218_RS07905 (1604707) 1604707..1605048 + 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
N7218_RS07910 (1605106) 1605106..1607610 - 2505 WP_000083947.1 copper-exporting P-type ATPase CopA -
N7218_RS07915 (1607872) 1607872..1608804 + 933 WP_000883041.1 glutaminase A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T259297 WP_000057523.1 NZ_CP104848:1604369-1604671 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT259297 WP_000806442.1 NZ_CP104848:1604707-1605048 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References