Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 4858172..4858774 Replicon chromosome
Accession NZ_CP104843
Organism Escherichia coli strain DFL.5

Toxin (Protein)


Gene name higB Uniprot ID U9XIS6
Locus tag N7217_RS23570 Protein ID WP_000897305.1
Coordinates 4858463..4858774 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag N7217_RS23565 Protein ID WP_000356397.1
Coordinates 4858172..4858462 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N7217_RS23545 (4854674) 4854674..4855576 + 903 WP_000331377.1 formate dehydrogenase O subunit beta -
N7217_RS23550 (4855573) 4855573..4856208 + 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
N7217_RS23555 (4856205) 4856205..4857134 + 930 WP_000027720.1 formate dehydrogenase accessory protein FdhE -
N7217_RS23560 (4857349) 4857349..4857567 - 219 WP_001298592.1 CopG family transcriptional regulator -
N7217_RS23565 (4858172) 4858172..4858462 - 291 WP_000356397.1 NadS family protein Antitoxin
N7217_RS23570 (4858463) 4858463..4858774 - 312 WP_000897305.1 hypothetical protein Toxin
N7217_RS23575 (4859003) 4859003..4859911 + 909 WP_001318161.1 alpha/beta hydrolase -
N7217_RS23580 (4859975) 4859975..4860916 - 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
N7217_RS23585 (4860961) 4860961..4861398 - 438 WP_000560983.1 D-aminoacyl-tRNA deacylase -
N7217_RS23590 (4861395) 4861395..4862267 - 873 WP_000920762.1 virulence factor BrkB family protein -
N7217_RS23595 (4862261) 4862261..4862860 - 600 WP_001314324.1 glucose-1-phosphatase -
N7217_RS23600 (4862959) 4862959..4863744 - 786 WP_000059679.1 DeoR/GlpR family DNA-binding transcription regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12190.19 Da        Isoelectric Point: 9.7791

>T259250 WP_000897305.1 NZ_CP104843:c4858774-4858463 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10846.43 Da        Isoelectric Point: 10.8907

>AT259250 WP_000356397.1 NZ_CP104843:c4858462-4858172 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y7I7

Antitoxin

Source ID Structure

References