Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3908265..3908959 Replicon chromosome
Accession NZ_CP104843
Organism Escherichia coli strain DFL.5

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag N7217_RS18965 Protein ID WP_001263491.1
Coordinates 3908265..3908663 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag N7217_RS18970 Protein ID WP_000554755.1
Coordinates 3908666..3908959 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N7217_RS18935 (3903527) 3903527..3904984 + 1458 WP_022645227.1 cytosol nonspecific dipeptidase -
N7217_RS18940 (3904993) 3904993..3905274 + 282 WP_022645226.1 hypothetical protein -
N7217_RS18945 (3905291) 3905291..3905800 - 510 WP_001361775.1 metal-dependent hydrolase -
N7217_RS18950 (3905862) 3905862..3906476 - 615 WP_022645225.1 peptide chain release factor H -
N7217_RS18955 (3906473) 3906473..3907612 - 1140 WP_022645224.1 RNA ligase RtcB family protein -
N7217_RS18960 (3907803) 3907803..3908255 - 453 WP_023909048.1 GNAT family N-acetyltransferase -
N7217_RS18965 (3908265) 3908265..3908663 - 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
N7217_RS18970 (3908666) 3908666..3908959 - 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
N7217_RS18975 (3909011) 3909011..3910066 - 1056 WP_022645222.1 DNA polymerase IV -
N7217_RS18980 (3910137) 3910137..3910922 - 786 WP_072224360.1 putative lateral flagellar export/assembly protein LafU -
N7217_RS18985 (3910894) 3910894..3912606 + 1713 Protein_3720 flagellar biosynthesis protein FlhA -
N7217_RS18990 (3912704) 3912704..3913477 - 774 WP_022645219.1 C40 family peptidase -
N7217_RS18995 (3913663) 3913663..3913923 + 261 WP_000729708.1 type II toxin-antitoxin system antitoxin DinJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T259243 WP_001263491.1 NZ_CP104843:c3908663-3908265 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT259243 WP_000554755.1 NZ_CP104843:c3908959-3908666 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References