Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 3708094..3708773 Replicon chromosome
Accession NZ_CP104843
Organism Escherichia coli strain DFL.5

Toxin (Protein)


Gene name higB Uniprot ID A0A0A1A1R1
Locus tag N7217_RS18020 Protein ID WP_000057541.1
Coordinates 3708471..3708773 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag N7217_RS18015 Protein ID WP_000806442.1
Coordinates 3708094..3708435 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N7217_RS18005 (3704337) 3704337..3705269 - 933 WP_022645289.1 glutaminase A -
N7217_RS18010 (3705532) 3705532..3708036 + 2505 WP_038431953.1 copper-exporting P-type ATPase CopA -
N7217_RS18015 (3708094) 3708094..3708435 - 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
N7217_RS18020 (3708471) 3708471..3708773 - 303 WP_000057541.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
N7217_RS18025 (3708906) 3708906..3709700 + 795 WP_022645287.1 TraB/GumN family protein -
N7217_RS18030 (3709904) 3709904..3710383 + 480 WP_000186638.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
N7217_RS18035 (3710420) 3710420..3712072 - 1653 WP_000771748.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
N7217_RS18040 (3712290) 3712290..3713510 + 1221 WP_022645286.1 fosmidomycin MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11795.37 Da        Isoelectric Point: 10.2638

>T259241 WP_000057541.1 NZ_CP104843:c3708773-3708471 [Escherichia coli]
MAQKNNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT259241 WP_000806442.1 NZ_CP104843:c3708435-3708094 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A1A1R1

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References